Zeng Xiangwei, Liu Lanlan, Hao Ruijun, Han Chunyan
College of Wildlife Resources, Northeast Forestry University, Harbin, China.
College of Basic Medical Science, Heilongjiang University of Chinese Medicine, Harbin, China.
PLoS One. 2014 Apr 14;9(4):e94980. doi: 10.1371/journal.pone.0094980. eCollection 2014.
To assess the status of avian leukosis virus subgroup J (ALV-J) in wild ducks in China, we examined samples from 528 wild ducks, representing 17 species, which were collected in China over the past 3 years. Virus isolation and PCR showed that 7 ALV-J strains were isolated from wild ducks. The env genes and the 3'UTRs from these isolates were cloned and sequenced. The env genes of all 7 wild duck isolates were significantly different from those in the prototype strain HPRS-103, American strains, broiler ALV-J isolates and Chinese local chicken isolates, but showed close homology with those found in some layer chicken ALV-J isolates and belonged to the same group. The 3'UTRs of 7 ALV-J wild ducks isolates showed close homology with the prototype strain HPRS-103 and no obvious deletion was found in the 3'UTR except for a 1 bp deletion in the E element that introduced a binding site for c-Ets-1. Our study demonstrated the presence of ALV-J in wild ducks and investigated the molecular characterization of ALV-J in wild ducks isolates.
为评估中国野鸭中禽白血病病毒J亚群（ALV-J）的感染状况，我们检测了过去3年在中国采集的代表17个物种的528只野鸭的样本。病毒分离和PCR检测显示，从野鸭中分离出7株ALV-J毒株。对这些分离株的env基因和3'UTR进行了克隆和测序。所有7株野鸭分离株的env基因与原型毒株HPRS-103、美国毒株、肉鸡ALV-J分离株及中国地方鸡分离株的env基因存在显著差异，但与一些蛋鸡ALV-J分离株中的env基因具有密切同源性，属于同一组。7株ALV-J野鸭分离株的3'UTR与原型毒株HPRS-103具有密切同源性，除E元件中有1个碱基缺失并引入了c-Ets-1结合位点外，3'UTR未发现明显缺失。我们的研究证实了野鸭中存在ALV-J，并对野鸭分离株中ALV-J的分子特征进行了研究。
