Theodoro Leticia Helena, Caiado Roberto Coelho, Longo Mariéllen, Novaes Vivian Cristina Noronha, Zanini Naida Assem, Ervolino Edilson, de Almeida Juliano Milanezi, Garcia Valdir Gouveia
Group of Research and Study on Laser in Dentistry (GEPLO), Department of Surgery and Integrated Clinic, Division of Periodontics, University Estadual Paulista (UNESP), Araçatuba, SP, Brazil,
Lasers Med Sci. 2015 May;30(4):1209-18. doi: 10.1007/s10103-014-1575-7. Epub 2014 Apr 15.
The aim of this study was to evaluate the effects of the use of a high-power gallium-aluminum-arsenide diode laser (GaAlAs; 808 nm, 1 W, 20 s, 20 Hz, 10 J) alone or as adjunctive therapy to scaling and root planing in the treatment of induced periodontitis in rats. Periodontitis was induced by placing a ligature around the mandibular first molar of 60 rats. After 7 days, the ligature was removed and the animals were divided into four groups as follows: C (control), no periodontal treatment; SRP, scaling and root planing (SRP); DL, diode laser (DL) irradiation treatment; and SRP/DL, both SRP and DL irradiation treatment. Five animals from each group were euthanized at 7, 15, and 30 days posttreatment. The effectiveness of the treatments was evaluated in the furcation area using histopathological analysis, histometric analysis of alveolar bone loss (ABL), and immunohistochemical detection of tartrate-resistant acid phosphatase (TRAP), runt-related transcription factor 2 (RUNX2), and osteocalcin (OCN). DL, alone or in combination with adjunctive therapy to SRP in the treatment of experimental periodontitis, resulted in a decreased local inflammatory response. At 7-days posttreatment, the DL and SRP/DL groups had fewer TRAP-positive cells and more RUNX2-positive cells. There was greater OCN immunolabeling in the DL group than in the C and SRP groups at 15 days. There was less ABL in the DL and SRP/DL groups at 15 and 30 days. In conclusion, DL was effective in the treatment of ligature-induced periodontitis in rats, both when used alone and when used as adjunctive therapy to SRP.
本研究旨在评估单独使用高功率砷化镓铝二极管激光（GaAlAs；808纳米，1瓦，20秒，20赫兹，10焦耳）或作为龈下刮治和根面平整辅助治疗手段，对大鼠实验性牙周炎的治疗效果。通过在60只大鼠的下颌第一磨牙周围放置结扎丝来诱导牙周炎。7天后，移除结扎丝，将动物分为四组：C组（对照组），不进行牙周治疗；SRP组，龈下刮治和根面平整（SRP）；DL组，二极管激光（DL）照射治疗；SRP/DL组，龈下刮治和根面平整联合DL照射治疗。每组5只动物在治疗后7天、15天和30天实施安乐死。使用组织病理学分析、牙槽骨吸收（ABL）的组织计量学分析以及抗酒石酸酸性磷酸酶（TRAP）、 runt相关转录因子2（RUNX2）和骨钙素（OCN）的免疫组织化学检测，在根分叉区域评估治疗效果。单独使用DL或与SRP辅助治疗联合用于实验性牙周炎的治疗时，均可降低局部炎症反应。治疗后7天，DL组和SRP/DL组的TRAP阳性细胞较少，RUNX2阳性细胞较多。15天时，DL组的OCN免疫标记比C组和SRP组更多。15天和30天时，DL组和SRP/DL组的ABL较少。总之，DL单独使用或作为SRP的辅助治疗手段，对大鼠结扎丝诱导的牙周炎均有治疗效果。
