Nara N, Tohda S, Nagata K, Suzuki T, Yamashita Y
First Department of Internal Medicine, Tokyo Medical and Dental University, Japan.
Leukemia. 1989 Aug;3(8):572-7.
The effects of transforming growth factor-beta (TGF-beta) on the blast progenitors from nine acute myeloblastic leukemia patients were studied in methylcellulose and suspension cultures. Leukemic blast progenitors undergo terminal divisions with a limited differentiation in methylcellulose culture, making blast colonies. Blast progenitors can renew themselves. The self-renewal can be reflected by secondary colony formation after replating primary blast colonies in fresh methylcellulose media and by the exponential growth of clonogenic cells in suspension culture. TGF-beta suppressed primary and secondary colony-formation in methylcellulose culture. Furthermore, TGF-beta suppressed the recovery of clonogenic cells in suspension. The results indicate that TGF-beta is effective in inhibiting not only terminal divisions but also self-renewal of leukemic blast progenitors.
在甲基纤维素和悬浮培养中研究了转化生长因子-β（TGF-β）对9例急性髓性白血病患者的原始祖细胞的影响。白血病原始祖细胞在甲基纤维素培养中经历终末分裂且分化有限，形成原始集落。原始祖细胞能够自我更新。自我更新可通过将原始原始集落重新接种于新鲜甲基纤维素培养基后形成次级集落以及悬浮培养中克隆形成细胞的指数生长来反映。TGF-β抑制甲基纤维素培养中的初级和次级集落形成。此外，TGF-β抑制悬浮培养中克隆形成细胞的恢复。结果表明，TGF-β不仅能有效抑制白血病原始祖细胞的终末分裂，还能抑制其自我更新。
