Chen G J, Tomida M, Hozumi M, Nara N
Laboratory Medicine, Tokyo Medical and Dental University.
Jpn J Cancer Res. 1992 Dec;83(12):1341-6. doi: 10.1111/j.1349-7006.1992.tb02767.x.
We studied the effects of D-factor on the growth of leukemic blast progenitors from 15 patients with acute myeloblastic leukemia and two leukemia cell lines in methylcellulose and suspension cultures. When stimulated by granulocyte colony-stimulating factor (G-CSF), granulocyte-macrophage colony-stimulating factor or interleukin-3, leukemic blast progenitors undergo terminal division with limited differentiation in methylcellulose culture, forming blast colonies. Leukemic blast progenitors can renew themselves. The self-renewal can be detected as secondary colony formation after replating primary blast colonies in fresh methylcellulose media and by the growth of clonogenic cells in suspension culture. D-Factor suppressed primary and secondary colony formation in methylcellulose culture. Furthermore, D-factor suppressed clonogenic cell recovery in suspension culture. The suppression by D-factor of the growth of leukemic blast progenitors was not significantly dependent upon the colony-stimulating factors used as growth-stimulating factors. High concentration of G-CSF did not overcome the suppressive effect of D-factor. The results indicate that D-factor is effective in suppressing not only terminal division but also self-renewal of leukemic blast progenitors.
我们研究了D因子对15例急性髓细胞白血病患者的白血病原始祖细胞以及两种白血病细胞系在甲基纤维素和悬浮培养体系中生长的影响。当受到粒细胞集落刺激因子（G-CSF）、粒细胞-巨噬细胞集落刺激因子或白细胞介素-3刺激时，白血病原始祖细胞在甲基纤维素培养体系中经历终末分裂且分化有限，形成原始集落。白血病原始祖细胞能够自我更新。这种自我更新可通过将原始原始集落重新接种到新鲜的甲基纤维素培养基中后形成次级集落以及通过悬浮培养中克隆形成细胞的生长来检测。D因子抑制了甲基纤维素培养体系中原始集落和次级集落的形成。此外，D因子还抑制了悬浮培养中克隆形成细胞的恢复。D因子对白血病原始祖细胞生长的抑制作用并不显著依赖于用作生长刺激因子的集落刺激因子。高浓度的G-CSF并不能克服D因子的抑制作用。结果表明，D因子不仅能有效抑制白血病原始祖细胞的终末分裂，还能抑制其自我更新。
