Suzuki T, Nagata K, Murohashi I, Nara N
First Department of Internal Medicine, Tokyo Medical and Dental University, Japan.
Leukemia. 1988 Jun;2(6):358-62.
We studied the effects of recombinant human macrophage colony-stimulating factor (M-CSF) on the leukemic blast progenitors from 10 acute myeloblastic leukemia patients. Recombinant human (rh)M-CSF stimulated leukemic blast progenitors in methylcellulose in four patients, but the colonies by rhM-CSF were smaller in size and number than those by rh-granulocyte-CSF or human bladder carcinoma cell line 5637 conditioned medium. rhM-CSF did not increase the number of clonogenic cells in long-term suspension culture. The blast colony formation in methylcellulose and the exponential growth of clonogenic cells in long-term suspension culture are considered to reflect the terminal divisions and the self-renewal of blast progenitors, respectively. The results show that M-CSF stimulates terminal divisions weakly but does not stimulate self-renewal of leukemic blast progenitors. M-CSF did not induce differentiation of blasts either in methylcellulose or in suspension culture.
我们研究了重组人巨噬细胞集落刺激因子（M-CSF）对10例急性髓细胞白血病患者白血病原始祖细胞的影响。重组人（rh）M-CSF刺激了4例患者甲基纤维素中的白血病原始祖细胞，但rhM-CSF形成的集落大小和数量均小于rh粒细胞集落刺激因子或人膀胱癌细胞系5637条件培养基形成的集落。rhM-CSF未增加长期悬浮培养中克隆形成细胞的数量。甲基纤维素中的原始集落形成和长期悬浮培养中克隆形成细胞的指数生长分别被认为反映了原始祖细胞的终末分裂和自我更新。结果表明，M-CSF对终末分裂的刺激作用较弱，但不刺激白血病原始祖细胞的自我更新。M-CSF在甲基纤维素或悬浮培养中均未诱导原始细胞分化。
