Amanlou Massoud, Ghazi Moghadam Amin, Barazandeh Tehrani Maliheh, Souri Effat
Iran J Pharm Res. 2014 Winter;13(1):81-6.
In this study a sensitive, simple and accurate spectrophotometric method was suggested for determination of tamsulosin in bulk powder and pharmaceutical dosage form based on the formation of an ion-pair complex between the drug and bromocresol green in a buffer solution at pH 3.5. The formed yellow color complex was extracted with chloroform and measured at 415 nm. The optimum reaction conditions such as pH, reagent amount, extracting solvent and the stoichiometry of the ion-pair complex were investigated. Under the optimized conditions, the Beer's law was obeyed in the concentration range of 1-160 g/mL with acceptable correlation coefficient (r(2) > 0.9997) and precision (CV < 3%) and accuracy (error < 2%). The proposed method was successfully used for the determination of tamsulosin in pharmaceutical capsule with nosignificant interferences of excipients.
本研究提出了一种灵敏、简便且准确的分光光度法，用于测定原料药粉末和药物剂型中的坦索罗辛。该方法基于在pH 3.5的缓冲溶液中，药物与溴甲酚绿形成离子对络合物。所形成的黄色络合物用氯仿萃取，并在415 nm处进行测定。研究了最佳反应条件，如pH、试剂量、萃取溶剂和离子对络合物的化学计量关系。在优化条件下，在1 - 160 μg/mL的浓度范围内符合比尔定律，具有可接受的相关系数（r² > 0.9997）、精密度（CV < 3%）和准确度（误差 < 2%）。所提出的方法成功用于测定药物胶囊中的坦索罗辛，辅料无明显干扰。
