Souri Effat, Rahimi Aghil, Shabani Ravari Nazanin, Barazandeh Tehrani Maliheh
Department of Medicinal Chemistry, Faculty of Pharmacy and Pharmaceutical Research Center, Tehran University of Medical Sciences, Tehran, Iran.
Iran J Pharm Res. 2015 Spring;14(2):435-42.
A mixture of acetaminophen, diphenhydramine hydrochloride and pseudoephedrine hydrochloride is used for the symptomatic treatment of common cold. In this study, a derivative spectrophotometric method based on zero-crossing technique was proposed for simultaneous determination of acetaminophen, diphenhydramine hydrochloride and pseudoephedrine hydrochloride. Determination of these drugs was performed using the (1)D value of acetaminophen at 281.5 nm, (2)D value of diphenhydramine hydrochloride at 226.0 nm and (4)D value of pseudoephedrine hydrochloride at 218.0 nm. The analysis method was linear over the range of 5-50, 0.25-4, and 0.5-5 µg/mL for acetaminophen, diphenhydramine hydrochloride and pseudoephedrine hydrochloride, respectively. The within-day and between-day CV and error values for all three compounds were within an acceptable range (CV<2.2% and error<3%). The developed method was used for simultaneous determination of these drugs in pharmaceutical dosage forms and no interference from excipients was observed.
对乙酰氨基酚、盐酸苯海拉明和盐酸伪麻黄碱的混合物用于普通感冒的对症治疗。在本研究中，提出了一种基于零交叉技术的导数分光光度法，用于同时测定对乙酰氨基酚、盐酸苯海拉明和盐酸伪麻黄碱。使用（1）对乙酰氨基酚在281.5nm处的D值、（2）盐酸苯海拉明在226.0nm处的D值和（4）盐酸伪麻黄碱在218.0nm处的D值对这些药物进行测定。该分析方法对对乙酰氨基酚、盐酸苯海拉明和盐酸伪麻黄碱的线性范围分别为5 - 50、0.25 - 4和0.5 - 5μg/mL。所有三种化合物的日内和日间CV及误差值均在可接受范围内（CV<2.2%且误差<3%）。所建立的方法用于同时测定药物剂型中的这些药物，未观察到辅料的干扰。
