Tharavichitkul Prasit, Wongsawan Kanreuthai, Takenami Naoki, Pruksakorn Sumalee, Fongcom Achara, Gottschalk Marcelo, Khanthawa Banyong, Supajatura Volaluk, Takai Shinji
Department of Microbiology, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand.
Department of Veterinary Biosciences and Public Health, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai 50200, Thailand.
J Pathog. 2014;2014:350416. doi: 10.1155/2014/350416. Epub 2014 Mar 6.
Streptococcus suis infection is a severe zoonotic disease commonly found in Northern Thailand where people often consume raw pork and/or pig's blood. The most frequent clinical presentations are meningitis, sepsis, and endocarditis with higher rate of mortality and hearing loss sequelae. To clarify the correlation between pulsed-field gel electrophoresis (PFGE) groups and mrp/epf/sly genotypes of S. suis serotype 2, 62 patient and 4 healthy pig isolates from Northern Thailand were studied. By PFGE analysis, at 66% homology, most human isolates (69.4%) and 1 pig isolate were in group A, whereas 14.5% of human isolates and 3 out of 4 pig isolates were in group D. According to mrp/epf/sly genotypes, 80.6% of human isolates were identified in mrp (+) epf (-) sly (-) and only 12.9% were in mrp (-) epf (-) sly (+) genotypes; in contrast, 1 and 3 pig isolates were detected in these two genotypes, respectively. Interestingly, all isolates of S. suis serotype 2 classified in PFGE groups A, B, and E were set in mrp (+) epf (-) sly (-) genotypes. These data show a close correlation between PFGE groups and mrp/epf/sly genotypes of human S. suis serotype 2.
猪链球菌感染是一种严重的人畜共患病，常见于泰国北部，当地居民经常食用生猪肉和/或猪血。最常见的临床表现为脑膜炎、败血症和心内膜炎，死亡率和听力丧失后遗症发生率较高。为了阐明猪链球菌2型脉冲场凝胶电泳（PFGE）分组与mrp/epf/sly基因型之间的相关性，对来自泰国北部的62株患者分离株和4株健康猪分离株进行了研究。通过PFGE分析，在66%的同源性水平下，大多数人类分离株（69.4%）和1株猪分离株属于A组，而14.5%的人类分离株和4株猪分离株中的3株属于D组。根据mrp/epf/sly基因型，80.6%的人类分离株为mrp（+）epf（-）sly（-）基因型，只有12.9%为mrp（-）epf（-）sly（+）基因型；相比之下，这两种基因型中分别检测到1株和3株猪分离株。有趣的是，PFGE分组为A、B和E组的所有猪链球菌2型分离株均为mrp（+）epf（-）sly（-）基因型。这些数据表明，人类猪链球菌2型的PFGE分组与mrp/epf/sly基因型之间存在密切相关性。
