Water Environ Res. 2014 Mar;86(3):258-68. doi: 10.2175/106143013x13807328849297.
Rhizospheric microorganisms are important for environmental conservancy. The constancy and variability of the microorganisms in the rhizosphere of Phragmites australis in relation to the spatiotemporal variations in wetland ecosystems were studied. During the peak and trough of the vegetative period of the Phragmites australis growing across the hydrologic gradients of the Sun Island Wetland, Biolog and denaturing gradient gel electrophoresis (DGGE) were used to investigate the rhizospheric microbial characteristics. Both methods demonstrated that the microbial activity, richness, and diversity decreased from summer to autumn. However, these properties did not show significant correlation with hydrologic gradient, except that the genetic richness and diversity of the fungi decreased with it. Cluster analysis also demonstrated that the rhizospheric microbial community seemed to be largely affected by a vegetative period. In addition, this research was extended to a broader range of determining the universal microorganisms, which showed notable adaptability.
根际微生物对于环境保护至关重要。本研究旨在探讨芦苇根际微生物与湿地生态系统时空变化的关系。在太阳岛湿地水文梯度上生长的芦苇营养期的高峰期和低谷期，利用 Biolog 和变性梯度凝胶电泳（DGGE）技术研究了根际微生物特性。这两种方法都表明，微生物活性、丰富度和多样性从夏季到秋季逐渐降低。然而，这些特性与水文梯度没有显著相关性，除了真菌的遗传丰富度和多样性随水文梯度而降低。聚类分析也表明，根际微生物群落似乎主要受到营养期的影响。此外，本研究还扩展到了更广泛的确定普遍微生物的范围，这些微生物表现出显著的适应性。
