Tanaka Takashi S T, Irbis Chagan, Wang Pengyun, Inamura Tatsuya
Faculty of Life Science and Technology, Kunming University of Science and Technology, 727 Jingming Road (South), Chenggong New District, Kunming, Yunnan 650500, China Graduate School of Agriculture, Kyoto University, Oiwake Kitashirakawa Sakyo, Kyoto 606-8502, Japan.
Faculty of Life Science and Technology, Kunming University of Science and Technology, 727 Jingming Road (South), Chenggong New District, Kunming, Yunnan 650500, China
FEMS Microbiol Ecol. 2015 Feb;91(2):1-10. doi: 10.1093/femsec/fiu019. Epub 2014 Dec 10.
Plant harvest is one of the most important management practices in constructed wetlands. In this study, we evaluated the impact of harvesting Phragmites australis (Cav.) Trin. ex Steudel on the activity and community structure of nitrifiers and denitrifiers in a free-water surface constructed wetland. The nitrifiers were targeted using bacterial and archaeal-amoA that encode ammonia monooxygenase, and the denitrifiers were targeted using nirK and nirS that encode the nitrite reductase. The community structures were evaluated using denaturing gradient gel electrophoresis. The potential nitrification and nitrate reduction rates were shown to be significantly higher in the harvested plant rhizosphere than in a non-harvested control plot. The potential nitrification rate positively correlated with the potential nitrate reduction rate and influenced the community structure of nirK. In addition, plant canopy developed differently after harvest and simultaneously changed the microclimate beneath the plant community. These results suggest that plant harvest management could change subsequent plant development and associated microenvironments, thereby affecting the function and community structure of nitrifiers and denitrifiers. Our study highlights the importance of plant harvest management within constructed wetlands to regulate the functions of nitrification and denitrification.
植物收割是人工湿地中最重要的管理措施之一。在本研究中，我们评估了收割芦苇（Phragmites australis (Cav.) Trin. ex Steudel）对自由水面人工湿地中硝化细菌和反硝化细菌的活性及群落结构的影响。利用编码氨单加氧酶的细菌和古菌amoA基因靶向硝化细菌，利用编码亚硝酸还原酶的nirK和nirS基因靶向反硝化细菌。采用变性梯度凝胶电泳评估群落结构。结果表明，收割植物的根际中潜在硝化速率和硝酸盐还原速率显著高于未收割的对照样地。潜在硝化速率与潜在硝酸盐还原速率呈正相关，并影响nirK的群落结构。此外，收割后植物冠层发育不同，同时改变了植物群落下方的微气候。这些结果表明，植物收割管理可能改变后续植物发育及相关微环境，从而影响硝化细菌和反硝化细菌的功能及群落结构。我们的研究强调了人工湿地中植物收割管理对调节硝化和反硝化功能的重要性。
