Water Environ Res. 2014 Mar;86(3):277-84. doi: 10.2175/106143013x13807328849495.
Naphthalene and BTEX (benzene, toluene, ethyl benzene, and xylene) are frequently detected toxic hydrocarbons in contaminated sites, which can easily enter the soil or groundwater system. To test the potential of treating these hydrocarbons in a conventional water resource recovery facility, municipal activated sludge was used as the seed and the bacteria successfully enriched using naphthalene or BTEX as the sole carbon source under aerobic conditions. The pseudo first order kinetic constant for naphthalene degradation by enriched bacteria was 14.053 L h(-1) g(-1). For BTEX degradation, kinetic constants of 0.234, 0.958, 1.212, and 0.455 L h(-1) g(-1)1' were obtained for benzene, toluene, ethyl benzene, and xylene, respectively, which collectively accounted for a total BTEX removal rate constant of 0.550 L h(-1) g(-1) . Through cloning and sequencing, Pseudomonas and Burkholderia were identified as the primary bacteria communities in the naphthalene degradation reactor, whereas for BTEX degradation, Pseudomonas and Acidovorax dominated in the reactor.
萘和 BTEX(苯、甲苯、乙苯和二甲苯)是污染场地中经常检测到的有毒碳氢化合物,它们很容易进入土壤或地下水系统。为了测试在常规水资源回收设施中处理这些碳氢化合物的潜力,采用城市活性污泥作为种子,并在好氧条件下成功地利用萘或 BTEX 作为唯一碳源富集细菌。富集细菌降解萘的拟一级动力学常数为 14.053 L h(-1) g(-1)。对于 BTEX 降解,苯、甲苯、乙苯和二甲苯的动力学常数分别为 0.234、0.958、1.212 和 0.455 L h(-1) g(-1),合计总 BTEX 去除率常数为 0.550 L h(-1) g(-1)。通过克隆和测序,鉴定出假单胞菌和伯克霍尔德菌是萘降解反应器中的主要细菌群落,而对于 BTEX 降解,假单胞菌和食酸菌在反应器中占主导地位。