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从含油污染土壤中分离出的假单胞菌 YNS1 对 BTEX 混合物的生物降解。

Biodegradation of BTEX mixture by Pseudomonas putida YNS1 isolated from oil-contaminated soil.

机构信息

Division of Biotechnology, Advanced Institute of Environment and Bioscience, College of Environmental and Bioresource Sciences, Chonbuk National University, Iksan 570-752, Korea.

出版信息

J Basic Microbiol. 2013 May;53(5):469-75. doi: 10.1002/jobm.201200067. Epub 2012 Aug 23.

DOI:10.1002/jobm.201200067
PMID:22915285
Abstract

The presence of mixed contaminants, such as BTEX (benzene, toluene, ethylbenzene and xylene isomers) can affect the biodegradation, fate and environmental impacts of each compound. To understand the influence of interactions among BTEX compounds on their biodegradation, four bacteria were isolated from oil-contaminated soil and assayed for BTEX biodegradation in vitro. The isolate exhibiting maximum biodegradation was identified as Pseudomonas putida based on the 16S rDNA sequence. The biodegradation of the BTEX compounds was greatly influenced by pH, temperature, and salinity. Substrate mixture studies (binary, tertiary and quaternary) revealed that the presence of toluene increased the biodegradation rate of benzene, ethylbenzene, and xylene.

摘要

混合污染物(如 BTEX 苯、甲苯、乙苯和二甲苯异构体)的存在会影响每种化合物的生物降解、归宿和环境影响。为了了解 BTEX 化合物之间相互作用对其生物降解的影响,从污染土壤中分离出 4 株细菌,并在体外测定其对 BTEX 的生物降解能力。根据 16S rDNA 序列,对具有最大生物降解能力的分离菌进行鉴定,鉴定为恶臭假单胞菌。BTEX 化合物的生物降解受 pH 值、温度和盐度的影响很大。基质混合物研究(二元、三元和四元)表明,甲苯的存在提高了苯、乙苯和二甲苯的生物降解速率。

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