Prenatal diagnosis of haemoglobinopathies by ion exchange HPLC of haemoglobins.

作者信息

Rouyer-Fessard P, Plassa F, Blouquit Y, Vidaud M, Varnavides L, Mibashan R S, Bellingham A, Beuzard Y

机构信息

INSERM U91, Laboratory of Clinical Biochemistry, Hôpital Henri Mondor, Creteil, France.

出版信息

Prenat Diagn. 1989 Jan;9(1):19-26. doi: 10.1002/pd.1970090104.

A new method, the cation exchange HPLC of haemoglobins, has been compared to the classical carboxymethyl cellulose (CMC) chromatography of globin chains for the prenatal diagnosis of beta thalassaemia and sickle cell disease. The two methods correlated highly. The HPLC procedure can use two independent and reliable means--optical density at 405 nm and radioactivity to determine the adult Hb/HbF+Fac ratio. The diagnosis is obtained in 15 min by cation exchange HPLC.