通过血红蛋白的生物 Rex 色谱法进行血红蛋白病的产前诊断。

Antenatal diagnosis of haemoglobinopathies by Biorex chromatography of haemoglobin.

作者信息

Blouquit Y, Beuzard Y, Varnavides L, Chabret C, Dumez Y, John P N, Rodeck C, White J M

出版信息

Br J Haematol. 1982 Jan;50(1):7-15. doi: 10.1111/j.1365-2141.1982.tb01885.x.

DOI:10.1111/j.1365-2141.1982.tb01885.x

Abstract

Biorex chromatograhpy of haemoglobin has been compared to the standard chromatographic separation of radioactive globin chains in 60 fetal blood samples obtained for the antenatal diagnosis of haemoglobinopathies. Biorex chromatography of haemoglobin permitted two measurements, the optical density at 418 nm and the radioactivity incorporated into fetal and adult haemoglobin. The two measurements were highly correlated (r2=0.96) and enabled a distinction between homozygous from heterozygous states of the diseases to be made, particularly in beta thalassaemia. A single column was used for 50 analyses. This fast and very sensitive method is proposed for the antenatal diagnosis of haemoglobinopathies using fetal blood.

摘要

已将血红蛋白的生物 Rex 色谱法与 60 份用于血红蛋白病产前诊断的胎儿血样中放射性珠蛋白链的标准色谱分离法进行了比较。血红蛋白的生物 Rex 色谱法允许进行两项测量，即 418 nm 处的光密度以及掺入胎儿和成人血红蛋白中的放射性。这两项测量高度相关（r2 = 0.96），能够区分疾病的纯合子状态与杂合子状态，尤其是在β地中海贫血中。一根柱子用于 50 次分析。建议采用这种快速且非常灵敏的方法，利用胎儿血样进行血红蛋白病的产前诊断。