Gebeile Rémi, Roger Christelle, Doche Christophe, Doche Laurence
Service d'hématologie et microbiologie, Laboratoire de biologie médicale, Centre hospitalier de Chambéry, France.
Service de biochimie, Laboratoire de biologie médicale, Centre hospitalier de Chambéry, France.
Ann Biol Clin (Paris). 2014 Mar-Apr;72(2):207-12. doi: 10.1684/abc.2014.0936.
The aim of the study is to specify the pre-analytical conditions required for Down's syndrome screening in first trimester by maternal serum markers. The concentration variation of both markers, hCGβ and PAPP-A, was analyzed at room temperature, at 4°C and during freezing-thawing cycles. Serum can be kept during 72h between 2 and 8°C with an acceptable bias of 5% for each marker. It can also undergo three freezing-thawing cycles without any variation of results. Preservation at room temperature (between 20 and 25°C) requires an analysis within 24h. From this study, writing recommendations enable to give a precise frame to pre-analytical processing and transport of blood samples, in a field where variations can lead to heavy therapeutic decisions.
本研究的目的是明确孕早期通过母体血清标志物进行唐氏综合征筛查所需的分析前条件。分析了两种标志物hCGβ和PAPP-A在室温、4°C以及冻融循环过程中的浓度变化。血清可在2至8°C下保存72小时，每种标志物的偏差可接受范围为5%。它也可经历三次冻融循环而结果无任何变化。在室温（20至25°C）下保存则需要在24小时内进行分析。通过本研究编写的建议能够为血液样本的分析前处理和运输提供精确框架，在该领域中，变化可能导致重大的治疗决策。
