Department of Radiology, The Second Hospital of Jilin University, Changchun, 130041 P. R. China.
Nanoscale. 2014 Jun 7;6(11):5770-6. doi: 10.1039/c3nr06292a. Epub 2014 Apr 16.
Photothermal therapy, as a physical therapeutic technique to kill cancer, has generated a great deal of interest. Photothermal agents hence play a critical role in this modern therapy. We report the use of transition metal oxides as photothermal agents based on PEGylated WO3-x nanoparticles. The well-prepared nanoparticles presented effective results during photothermal therapy both in vitro and in vivo by using near-IR laser irradiation (980 nm, 0.5 W cm(-2)). The tumor cells were effectively damaged using low power density during a short irradiation time without destroying healthy tissues. In vitro results of photothermal therapy with PEGylated WO3-x nanoparticles proved to be effective on 4T1 murine breast cancer cells via a confocal microscopy method and MTT assay. In vivo results were further confirmed by hematoxylin and eosin (H & E) histological staining. Additionally, PEGylated WO3-x nanoparticles were shown to be effective as a CT imaging contrast agent on a tumor-bearing mouse model. Our results suggest that this generation of PEGylated WO3-x nanoparticles can potentially be used in oncological CT imaging and photothermal therapy.
光热疗法作为一种杀死癌症的物理治疗技术，引起了极大的兴趣。光热剂在这种现代疗法中起着至关重要的作用。我们报告了使用过渡金属氧化物作为基于 PEG 化 WO3-x 纳米粒子的光热剂。通过使用近红外激光照射（980nm，0.5W/cm(-2)），精心制备的纳米粒子在体外和体内的光热治疗中均表现出了有效的效果。在短时间的低功率密度照射下，肿瘤细胞被有效地破坏，而健康组织不受破坏。通过共聚焦显微镜方法和 MTT 测定，PEG 化 WO3-x 纳米粒子的体外光热治疗结果证明对 4T1 鼠乳腺癌细胞有效。通过苏木精和伊红（H & E）组织学染色进一步证实了体内结果。此外，PEG 化 WO3-x 纳米粒子在荷瘤小鼠模型中也表现出有效的 CT 成像造影剂作用。我们的结果表明，这一代 PEG 化 WO3-x 纳米粒子有望用于肿瘤的 CT 成像和光热治疗。
