Grupe R, Ziska T, Richter A, Böhme B, Göres E
Institut für Pharmakologische Forschung, VEB des Pharmazeutischen Kombinates Germed Dresden, Berlin-Friedrichsfelde, DDR.
Biomed Biochim Acta. 1988;47(12):955-63.
Lipoxygenase inhibitors block the release of histamine from protaminesulfate-stimulated peritoneal mast cells of the rat in vitro. The order of this inhibitory activity of the agents does not correlate with the order of this inhibitory activity of the agents does not correlate with the order of their inhibitory activity against lipoxygenases from reticulocytes and peas. Antioxidants/radical scavengers, which do not inhibit lipoxygenase activity (mannitol, hydrochinone) block the protaminesulfate-activated degranulation of peritoneal rat mast cells, too. Therefore we assume that activated oxygen radicals are generated by stimulation of pRMZ with protaminesulfate. These oxygen radicals could be promotors for the histamine secretion of the peritoneal mast cells. Consequently, lipoxygenase inhibitors with radical scavenging properties may influence degranulation of peritoneal mast cells of the rat by two mechanisms, namely by reduction of the synthesis of 5-LOX-products (promotors of the histamine release) and by reduction of the toxic effects of activated oxygen radicals.
脂氧合酶抑制剂可在体外阻断硫酸鱼精蛋白刺激的大鼠腹膜肥大细胞释放组胺。这些药物的这种抑制活性顺序与它们对网织红细胞和豌豆中脂氧合酶的抑制活性顺序不相关。不抑制脂氧合酶活性的抗氧化剂/自由基清除剂（甘露醇、对苯二酚）也能阻断硫酸鱼精蛋白激活的大鼠腹膜肥大细胞脱颗粒。因此我们推测，硫酸鱼精蛋白刺激pRMZ会产生活性氧自由基。这些氧自由基可能是腹膜肥大细胞组胺分泌的促进剂。因此，具有自由基清除特性的脂氧合酶抑制剂可能通过两种机制影响大鼠腹膜肥大细胞的脱颗粒，即减少5-LOX产物（组胺释放促进剂）的合成以及减少活性氧自由基的毒性作用。
