Grupe R, Ziska T
Biopharm Co. Ltd., Berlin, Germany.
Agents Actions. 1994 Jun;41 Spec No:C34-6. doi: 10.1007/BF02007755.
We have investigated the effect of inhibitors of lipoxygenase (LOX), cyclooxygenase (COX) and dual inhibitors of both enzymes on the degranulation of peritoneal rat mast cells (pRMC) activated by different mechanisms. COX inhibitors weakly affected histamine secretion induced by A23187 and did not influence the histamine secretion induced by protamine in an isotonic medium but blocked protamine-induced release in a hypertonic medium. LOX- and dual-inhibitors inhibited secretion induced by A23187 and protamine under all conditions. As A23187 activates pRMC in an isotonic medium by Ca influx, and protamine acts in a hypertonic medium by mobilization of intracellular Ca and in an isotonic medium by both processes (this paper), LOX but not COX inhibitors, may block Ca influx, and both prevent Ca mobilization.
我们研究了脂氧合酶（LOX）抑制剂、环氧化酶（COX）抑制剂以及这两种酶的双重抑制剂对通过不同机制激活的大鼠腹膜肥大细胞（pRMC）脱颗粒的影响。COX抑制剂对A23187诱导的组胺分泌影响较弱，在等渗介质中对鱼精蛋白诱导的组胺分泌无影响，但在高渗介质中可阻断鱼精蛋白诱导的释放。LOX抑制剂和双重抑制剂在所有条件下均抑制A23187和鱼精蛋白诱导的分泌。由于A23187在等渗介质中通过钙内流激活pRMC，鱼精蛋白在高渗介质中通过动员细胞内钙起作用，在等渗介质中则通过这两种过程起作用（本文），因此，LOX抑制剂而非COX抑制剂可能阻断钙内流，两者均可防止钙动员。
