细胞学和微生物学方法在新型隐球菌脑膜炎中的诊断价值

Diagnostic value of cytological and microbiological methods in cryptococcal meningitis.

作者信息

Zhang M, Li J C, Lin H, Zhang W, Lin M, Wu L, Liu W, Mu J S, Ye J X, Cui X P

机构信息

Department of Neurology, Fuzhou Dongfang Hospital, Fujian Medical University, Fuzhou, China.

Department of Emergency Medicine, Fuzhou Dongfang Hospital, Fujian Medical University, Fuzhou, China

出版信息

Genet Mol Res. 2014 Mar 26;13(4):9253-61. doi: 10.4238/2014.March.26.3.

DOI:10.4238/2014.March.26.3

PMID:24737516

Abstract

The aim of this study was to investigate diagnostic methods for cryptococcal meningitis (CM). A retrospective analysis was conducted for 31 patients with CM confirmed by etiologic detection of cerebrospinal fluid in our hospital in the past 5 years. Nineteen cases in 31 patients were confirmed with CM in the first diagnosis, with a misdiagnosis rate of 38.7%. The positive rates of cryptococcus detection in cerebrospinal fluid with May-Grünwald-Giemsa (MGG)-, ink-, and Alcian blue-staining methods were 86.9, 70.9, and 80.6%, respectively. The misdiagnosis rate of CM is high during the early stage of disease. The total positive rate of cryptococcus diagnosis using the MGG-staining method was significantly higher than that using the ink-staining method. These results are important for diagnosing CM.

摘要

本研究旨在探讨隐球菌性脑膜炎（CM）的诊断方法。对我院过去5年经脑脊液病原学检测确诊的31例CM患者进行回顾性分析。31例患者中19例初诊即确诊为CM，误诊率为38.7%。采用迈-格-姬（MGG）染色法、墨汁染色法和阿尔辛蓝染色法检测脑脊液中隐球菌的阳性率分别为86.9%、70.9%和80.6%。CM在疾病早期误诊率较高。采用MGG染色法诊断隐球菌的总阳性率显著高于墨汁染色法。这些结果对CM的诊断具有重要意义。