de los Toyos J, Jalkanen S, Butcher E C
Department of Pathology, Stanford University Medical Center, CA 94305-5324.
Blood. 1989 Aug 1;74(2):751-60.
The homing of lymphocytes is controlled by interactions with high endothelial venules (HEV), specialized vessels that define sites of lymphocyte extravasation into lymph nodes and inflamed tissues. In humans, lymphocyte-HEV binding involves a lymphocyte surface glycoprotein (GP) of 85 to 95 kd (CD44, H-CAM), defined by monoclonal antibody (MoAb) Hermes-1. To define the expression of this homing-associated adhesion molecule during human lymphocyte development, we performed two-color immunofluorescence analyses of human bone marrow (BM), thymus, peripheral blood (PB), and tonsillar lymphocytes. The highest levels of Hermes-1 antigen are displayed by circulating B and T cells in the blood, which are uniformly positive and bear roughly twice the level of antigen present on mature lymphocytes within organized lymphoid tissues and BM. "Immature" (CD4+, CD8+) T cells in the thymus are Hermes-1lo to-, whereas thymocytes of mature phenotype (CD4+ or CD8+) are positive. The Hermes-1 antigen is present at high levels on the same population of thymocytes that bears high surface levels of CD3, a component of the T-cell antigen receptor complex, suggesting that levels of T-cell homing and antigen receptors characteristic of mature peripheral T cells appear coordinately during thymocyte maturation/selection. Essentially all T cells in the periphery are Hermes-1hi, including T blasts, and the homing-associated antigen is maintained at high levels on T cells stimulated in vitro by phytohemagglutinin (PHA) and on interleukin-2 (IL-2) maintained T-cell clones and lines. In contrast, although most resting IgD+ B cells are positive a significant fraction of B cells in tonsils are Hermes-1lo to-; these cells are predominantly PNAhi, IgD-, and CD20hi, a phenotype characteristic of sessile, activated B cells in germinal centers. In all lymphocyte populations examined, there is a linear correlation in staining for Hermes-1 and for Hermes-3, an antibody that defines a distinct functionally important epitope on this molecule. The results demonstrate a precise regulation of this homing-associated antigen during lymphocyte differentiation.
淋巴细胞的归巢受其与高内皮微静脉（HEV）相互作用的控制，HEV是一种特殊的血管，它界定了淋巴细胞外渗进入淋巴结和炎症组织的部位。在人类中，淋巴细胞与HEV的结合涉及一种85至95kd的淋巴细胞表面糖蛋白（GP，即CD44、H-CAM），该糖蛋白由单克隆抗体（MoAb）Hermes-1所界定。为了确定这种与归巢相关的黏附分子在人类淋巴细胞发育过程中的表达情况，我们对人类骨髓（BM）、胸腺、外周血（PB）和扁桃体淋巴细胞进行了双色免疫荧光分析。血液中循环的B细胞和T细胞显示出最高水平的Hermes-1抗原，它们均呈阳性，其抗原水平大约是有组织的淋巴组织和骨髓中成熟淋巴细胞上抗原水平的两倍。胸腺中的“未成熟”（CD4+ CD8+）T细胞Hermes-1表达低至阴性，而成熟表型（CD4+或CD8+）的胸腺细胞则呈阳性。Hermes-1抗原在与T细胞抗原受体复合物的一个成分CD3表面水平高的同一群胸腺细胞上高水平存在，这表明成熟外周T细胞特有的T细胞归巢水平和抗原受体水平在胸腺细胞成熟/选择过程中协同出现。外周基本上所有的T细胞都是Hermes-1高表达，包括T母细胞，并且在体外受植物血凝素（PHA）刺激的T细胞以及在白细胞介素-2（IL-2）维持的T细胞克隆和系中，与归巢相关的抗原都维持在高水平。相比之下，虽然大多数静息的IgD+B细胞呈阳性，但扁桃体中的相当一部分B细胞Hermes-1表达低至阴性；这些细胞主要是PNA高表达、IgD阴性和CD20高表达，这是生发中心中固着的活化B细胞的表型特征。在所有检测的淋巴细胞群体中，Hermes-1染色与Hermes-3染色呈线性相关，Hermes-3是一种能界定该分子上一个独特的功能重要表位的抗体。结果表明在淋巴细胞分化过程中对这种与归巢相关的抗原存在精确调控。
