Li Zhi, Pan Li-Li, Zhang Fei-Long, Wang Zhiyuan, Shen Ying-Ying, Zhang Zhen-Zhong
J Nanosci Nanotechnol. 2014 Jun;14(6):4513-8. doi: 10.1166/jnn.2014.8242.
The properties of an ideal photosensitizer are water solubility, low cytotoxicity in the dark, high ability to produce reactive oxygen species (ROS). The characteristics of water-soluble fullerene (C60) amino acid nanoparticles as a photosensitizer were evaluated. C60 modified with l-phenylalanine (C60-phe) or glycine (C60-gly) was very efficient to carry out photodynamic activity leading to cleavage of plasmid DNA in vitro. These C60 amino acid nanoparticles were the most active photosensitizer against human Liver cancer cells and induced cancer cells apoptosis after illumination. However, these derivatives exhibited no significant cytotoxicity in dark. It produced diffuse intracellular fluorescence when 2',7'-dichlorfluorescein-diacetate (DCFH-DA) was added as an ROS probe, suggesting phototoxicity of these derivatives related with the generation of intracellular ROS. These findings indicate that these fullerene derivatives may be excellent candidate PDT enhancing agents.
理想的光敏剂特性包括水溶性、在黑暗中细胞毒性低、产生活性氧（ROS）的能力强。评估了水溶性富勒烯（C60）氨基酸纳米颗粒作为光敏剂的特性。用L-苯丙氨酸（C60-苯丙氨酸）或甘氨酸（C60-甘氨酸）修饰的C60在体外进行光动力活性以导致质粒DNA切割方面非常有效。这些C60氨基酸纳米颗粒是针对人肝癌细胞最具活性的光敏剂，光照后可诱导癌细胞凋亡。然而，这些衍生物在黑暗中未表现出明显的细胞毒性。当加入2',7'-二氯荧光素二乙酸酯（DCFH-DA）作为ROS探针时，它产生弥漫性细胞内荧光，表明这些衍生物的光毒性与细胞内ROS的产生有关。这些发现表明这些富勒烯衍生物可能是优秀的光动力疗法增强剂候选物。
