牛晶状体23kDa、21kDa和19kDa内在膜蛋白具有相同的氨基末端氨基酸序列。
Bovine lens 23, 21 and 19 kDa intrinsic membrane proteins have an identical amino-terminal amino acid sequence.
作者信息
Rao G N, Gutekunst K A, Church R L
机构信息
Department of Ophthalmology, Emory University School of Medicine, Atlanta, GA 30322.
出版信息
FEBS Lett. 1989 Jul 3;250(2):483-6. doi: 10.1016/0014-5793(89)80781-1.
We have isolated bovine lens intrinsic membrane proteins (MP) having molecular masses of 19, 21 and 23 kDa. Limited amino acid sequence analysis of the amino-terminal portion of each of these polypeptides revealed a 100% homology in sequence for the number of residues determined (20 amino acids). Northern blot analysis of bovine lens mRNA using a labeled antisense oligonucleotide probe common to the amino acid sequence of these three peptides revealed a single band having an apparent molecular size of 0.8 kb. Taken together, these findings suggest a genetic commonality between these polypeptides.
我们已经分离出了分子量分别为19 kDa、21 kDa和23 kDa的牛晶状体内在膜蛋白（MP）。对这些多肽各自氨基末端部分进行的有限氨基酸序列分析显示，在所测定的残基数量（20个氨基酸）范围内，序列同源性为100%。使用针对这三种肽氨基酸序列的标记反义寡核苷酸探针，对牛晶状体mRNA进行Northern印迹分析，结果显示有一条表观分子大小为0.8 kb的单条带。综合这些发现表明，这些多肽之间存在遗传共性。
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