使用简单设备通过脉冲场凝胶电泳轻松清洗裂解细胞塞进行细菌分型。
Easy washing of lysed cell plugs for bacterial typing by pulsed-field gel electrophoresis using simple equipment.
机构信息
Department of Health Science, Fukuoka Institute of Health and Environmental Sciences, 39 Mukaizano, Dazaifu, Fukuoka 818-0135, Japan.
Department of Health Science, Fukuoka Institute of Health and Environmental Sciences, 39 Mukaizano, Dazaifu, Fukuoka 818-0135, Japan; Department of Health Sciences, Faculty of Medical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan.
出版信息
J Microbiol Methods. 2014 Jun;101:67-9. doi: 10.1016/j.mimet.2014.04.001. Epub 2014 Apr 13.
We designed and tested equipment to wash plugs following cell lysis in pulsed-field gel electrophoresis (PFGE). Our system can wash 30 plugs simultaneously in 1h using 15L of Tris-EDTA buffer, which makes plug washing for PFGE less labor-intensive.
我们设计并测试了用于在脉冲场凝胶电泳（PFGE）中细胞裂解后清洗插头的设备。我们的系统可以在 1 小时内使用 15L 的 Tris-EDTA 缓冲液同时清洗 30 个插头，这使得 PFGE 的插头清洗不再那么费力。
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