Doran Geraldine, Morris Dearbhaile, O'Hare Colette, DeLappe Niall, Bradshaw Bernard, Corbett-Feeney Geraldine, Cormican Martin
National Salmonella Reference Laboratory, University College Hospital, Galway, Ireland.
Appl Environ Microbiol. 2005 Dec;71(12):8236-40. doi: 10.1128/AEM.71.12.8236-8240.2005.
Salmonella enterica serovar Typhimurium is frequently isolated from humans and animals. Phage typing is historically the first-line reference typing technique in Europe. It is rapid and convenient for laboratories with appropriate training and experience, and costs of consumables are low. Phage typing and pulsed-field gel electrophoresis (PFGE) were performed on 503 isolates of serovar Typhimurium. Twenty-nine phage types and 53 PFGE patterns were observed. Most isolates of phage types DT104, DT104b, and U310 are not distinguishable from other members of their phage type by PFGE. By contrast, PFGE of isolates of phage types DT193 and U302 shows great heterogeneity. Analysis of experience with PFGE and phage typing can facilitate the selective application of PFGE to maximize the yield of epidemiologically relevant additional information while controlling costs.
肠炎沙门氏菌鼠伤寒血清型经常从人和动物中分离出来。在欧洲，噬菌体分型在历史上是一线参考分型技术。对于经过适当培训且有经验的实验室来说，它快速且方便，并且耗材成本低。对503株鼠伤寒血清型菌株进行了噬菌体分型和脉冲场凝胶电泳（PFGE）。观察到29种噬菌体类型和53种PFGE图谱。噬菌体类型DT104、DT104b和U310的大多数菌株通过PFGE无法与它们噬菌体类型的其他成员区分开来。相比之下，噬菌体类型DT193和U302菌株的PFGE显示出很大的异质性。对PFGE和噬菌体分型经验的分析有助于有选择地应用PFGE，以在控制成本的同时最大化流行病学相关附加信息的产出。
