大鼠α2-巨球蛋白编码基因的结构

Structure of the rat alpha 2-macroglobulin-coding gene.

作者信息

Hattori M, Kusakabe S, Ohgusu H, Tsuchiya Y, Ito T, Sakaki Y

机构信息

Research Laboratory for Genetic Information, Kyushu, University, Fukuoka, Japan.

出版信息

Gene. 1989 Apr 30;77(2):333-40. doi: 10.1016/0378-1119(89)90081-4.

DOI:10.1016/0378-1119(89)90081-4

PMID:2473946

Abstract

Rat alpha 2-macroglobulin (alpha 2M) is an acute-phase protein, i.e., produced upon tissue inflammation. Genomic DNA clones covering the entire sequence of the alpha 2M gene were isolated and characterized by restriction mapping. Southern blotting and (partial) DNA sequencing. The rat alpha 2M gene is approx. 50 kb in length and consists of 36 exons ranging in size from 21 to 229 bp. Two functional domains, a bait region and a thiol ester site, are encoded by the exon 18 and 24, respectively. Several possible regulatory signals such as a TPA-inducible enhancer core, an identifier sequence, purine-pyrimidine alternative stretches and viral enhancer core sequences were identified. Several genomic DNA clones which cross-hybridized with the alpha 2M cDNA probe were also identified. Sequence analysis showed that they possessed sequences identical to a part of the rat alpha 1-inhibitor III cDNA and that they had a strikingly similar exon organization to the alpha 2M gene.

摘要

大鼠α2-巨球蛋白（α2M）是一种急性期蛋白，即在组织炎症时产生。分离出覆盖α2M基因全序列的基因组DNA克隆，并通过限制性图谱分析、Southern印迹分析和（部分）DNA测序对其进行表征。大鼠α2M基因长度约为50 kb，由36个外显子组成，大小从21到229 bp不等。两个功能结构域，一个诱饵区和一个硫酯位点，分别由第18和24外显子编码。鉴定出了几种可能的调控信号，如佛波酯（TPA）诱导的增强子核心、一个识别序列、嘌呤-嘧啶交替序列和病毒增强子核心序列。还鉴定出了几个与α2M cDNA探针交叉杂交的基因组DNA克隆。序列分析表明，它们具有与大鼠α1-抑制剂III cDNA的一部分相同的序列，并且它们的外显子组织与α2M基因惊人地相似。