Tsuchiya Y, Hattori M, Hayashida K, Ishibashi H, Okubo H, Sakaki Y
Research Laboratory for Genetic Information, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
Gene. 1987;57(1):73-80. doi: 10.1016/0378-1119(87)90178-8.
Rat alpha 2-macroglobulin (alpha 2M) is an acute-phase reactant, concentration of which in serum increases more than 100-fold in the course of inflammation. Glucocorticoid and some protein factors such as interleukin 1 (IL-1) have been known to be involved in the regulation of this plasma protein synthesis. To understand the regulatory mechanism of alpha 2M production at the molecular level, we isolated genomic DNA clones of rat alpha 2M gene and characterized the promoter region of the gene by comparing the nucleotide sequence with those of other acute-phase reactant genes. Several possible regulatory signals were identified. Particularly, a sequence (T/A)T(C/G)TGGGA(A/T) was found about at 170 bp upstream from a putative capping site, which was also found in the 5'flanking region of various acute-phase reactant genes.
大鼠α2-巨球蛋白（α2M）是一种急性期反应物，在炎症过程中其血清浓度会增加100倍以上。已知糖皮质激素和一些蛋白质因子如白细胞介素1（IL-1）参与这种血浆蛋白合成的调节。为了在分子水平上理解α2M产生的调节机制，我们分离了大鼠α2M基因的基因组DNA克隆，并通过将核苷酸序列与其他急性期反应物基因的序列进行比较来表征该基因的启动子区域。鉴定出了几种可能的调节信号。特别地，在假定的加帽位点上游约170bp处发现了一个序列（T/A）T（C/G）TGGGA（A/T），该序列也存在于各种急性期反应物基因的5'侧翼区域。
