[儿茶酚-O-甲基转移酶基因多态性与2型糖尿病关联的研究]

[Study on the association between catechol-O-methyltransferase gene polymorphisms and type 2 diabetes mellitus].

作者信息

Xiu Liangchang, Liu Weiwei, Zhou Shancun, Lin Meihua, Ouyang Ping, Qin Jiheng, Zhao Xiaolei, Kong Danli, Rao Shaoqi, Ding Yuanlin

机构信息

Department of Biostatistics, School of Public Health and Tropical Medicine, Southern Medical University, Guangzhou 510515, China; Department of Epidemiology and Medical Statistics, School of Public Health, Guangdong Medical College.

Department of Epidemiology and Medical Statistics, School of Public Health, Guangdong Medical College.

出版信息

Zhonghua Liu Xing Bing Xue Za Zhi. 2014 Feb;35(2):190-4.

PMID:24739563

Abstract

OBJECTIVE

To evaluate the association between the two single nucleotide polymorphisms located in catechol-O-methyltransferase (COMT) gene and type 2 diabetes mellitus (T2DM)in Han population in Guangdong province.

METHODS

Two tagSNPs (rs4646312 and rs4680) were picked out from COMT gene. Using the SNPscan(TM) Kit, SNP genotyping was then performed, in two cohorts, including 595 cases and 725 controls. Finally, Chi-square test, logistic regression model and other methods were employed for statistical analysis.

RESULTS

The frequencies of TT, CT and CC of rs4646312 appeared to be 304(51.1%), 234(39.3%)and 57 (8.6%) in cases, 323 (44.6%), 319 (44.0%) and 83(11.4%)in controls, respectively. The frequencies of GG,GA and AA of rs4680 were 311(52.4%), 236 (39.8%) and 46(7.8%)in cases, 417(57.7%), 265 (36.6%) and 41 (5.7%) in controls, respectively.

RESULTS

showed that SNP rs4646312 was significantly associated with T2DM both in allelic association analysis (P = 0.020,OR = 1.26, 95%CI:1.04-1.53)and in recessive model (P = 0.022, OR = 1.35, 95% CI:1.05-1.74)after adjustment for sex,BMI and TG. The association between rs4680 and T2DM was not significant, but BMI was remarkably different among the three genotypes of rs4680 after controlling for other factors.

CONCLUSION

SNP rs4646312 of COMT gene was associated with the increased risk of T2DM in Han population in Guangdong province. However, rs4680 was not significantly associated with T2DM.

摘要

目的

评估位于儿茶酚-O-甲基转移酶（COMT）基因上的两个单核苷酸多态性与广东省汉族人群2型糖尿病（T2DM）之间的关联。

方法

从COMT基因中挑选出两个标签单核苷酸多态性（rs4646312和rs4680）。然后使用SNPscan™试剂盒，在两个队列（包括595例病例和725例对照）中进行单核苷酸多态性基因分型。最后，采用卡方检验、逻辑回归模型等方法进行统计分析。

结果

rs4646312的TT、CT和CC基因型频率在病例组中分别为304（51.1%）、234（39.3%）和57（8.6%），在对照组中分别为323（44.6%）、319（44.0%）和83（11.4%）。rs4680的GG、GA和AA基因型频率在病例组中分别为311（52.4%）、236（39.8%）和46（7.8%），在对照组中分别为417（57.7%）、265（36.6%）和41（5.7%）。

结果

显示在调整性别、体重指数（BMI）和甘油三酯（TG）后，SNP rs4646312在等位基因关联分析（P = 0.020，比值比（OR）= 1.26，95%置信区间（CI）：1.04 - 1.53）和隐性模型（P = 0.022，OR = 1.35，95% CI：1.05 - 1.74）中均与T2DM显著相关。rs4680与T2DM之间的关联不显著，但在控制其他因素后，rs4680的三种基因型之间的BMI存在显著差异。