Indoxyl-tetranitro blue tetrazolium method for detection of alkaline phosphatase in immunohistochemistry.
作者信息
Brenan M, Bath M L
机构信息
Department of Immunology, John Curtin School of Medical Research, Australian National University, Canberra.
出版信息
J Histochem Cytochem. 1989 Aug;37(8):1299-301. doi: 10.1177/37.8.2474024.
PMID:2474024
Abstract
A sensitive method for detection of alkaline phosphatase in immunohistochemistry, using lymphoid cells, has been optimized. The conditions for staining are 0.23 mM 5-bromo-4-chloro-indoxyl phosphate, 0.55 mM tetranitro blue tetrazolium, 2.0 mM levamisole, 5.0 mM sodium azide, 10.0 mM magnesium chloride, and 0.15 mM 1-methoxyphenazine methosulfate dissolved in 100 mM Tris-HCl buffer, pH 9.5.
摘要
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