Weller P F, Monahan-Earley R A, Dvorak H F, Dvorak A M
Department of Medicine, Harvard Medical School, Boston, Massachusetts.
Am J Pathol. 1991 Jan;138(1):141-8.
Lipid bodies are non-membrane-bound cytoplasmic inclusions that are prominent in leukocytes engaged in inflammatory responses. As demonstrated by electron microscopic autoradiography, lipid bodies can serve as intracellular sites of 3H-arachidonic acid localization in eosinophils and other cells. To evaluate the role of lipid bodies as stores of esterified arachidonate, subcellular fractionation of lipid-body-rich human eosinophils was used to isolate lipid bodies free of other organelles. In lipid bodies isolated from 3H-arachidonate-labeled eosinophils, 3H-arachidonate was esterified almost totally in glycerolipids, predominantly in classes of phospholipids, including phosphatidyl-inositol and phosphatidylcholine. Lipid bodies, especially in leukocytes participating in inflammation, could represent intracellular sources of esterified arachidonate available for eicosanoid formation.
脂质体是无膜包裹的胞质内含物，在参与炎症反应的白细胞中很突出。如电子显微镜放射自显影所示，脂质体可作为嗜酸性粒细胞和其他细胞中3H-花生四烯酸的细胞内定位位点。为了评估脂质体作为酯化花生四烯酸储存库的作用，对富含脂质体的人嗜酸性粒细胞进行亚细胞分级分离，以分离出不含其他细胞器的脂质体。在从3H-花生四烯酸标记的嗜酸性粒细胞中分离出的脂质体中，3H-花生四烯酸几乎完全酯化在甘油脂质中，主要存在于磷脂类中，包括磷脂酰肌醇和磷脂酰胆碱。脂质体，尤其是参与炎症的白细胞中的脂质体，可能是可用于类花生酸形成的酯化花生四烯酸的细胞内来源。
