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紫外线-DNA修复酶T4内切核酸酶V封装于脂质体并递送至人类细胞。

Encapsulation of the UV-DNA repair enzyme T4 endonuclease V in liposomes and delivery to human cells.

作者信息

Ceccoli J, Rosales N, Tsimis J, Yarosh D B

机构信息

Applied Genetics Inc., Freeport, New York 11520.

出版信息

J Invest Dermatol. 1989 Aug;93(2):190-4. doi: 10.1111/1523-1747.ep12277569.

DOI:10.1111/1523-1747.ep12277569
PMID:2474027
Abstract

T4 endonuclease V, a pyrimidine-dimer-specific DNA repair enzyme, was encapsulated in liposomes under mild conditions. The encapsulated enzyme was active, and when applied to ultraviolet (UV)-irradiated human cells in culture, the liposomes increased incision of UV-irradiated cellular DNA, enhanced DNA repair replication, and enhanced survival of UV-irradiated cells. This method is a first step in a new approach for topical application of DNA repair enzymes to human skin to prevent skin cancer.

摘要

T4核酸内切酶V是一种嘧啶二聚体特异性DNA修复酶，在温和条件下被包裹于脂质体中。被包裹的酶具有活性，当应用于培养中的紫外线（UV）照射的人类细胞时，脂质体增加了UV照射的细胞DNA的切口，增强了DNA修复复制，并提高了UV照射细胞的存活率。该方法是将DNA修复酶局部应用于人类皮肤以预防皮肤癌的新方法的第一步。

相似文献

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Encapsulation of the UV-DNA repair enzyme T4 endonuclease V in liposomes and delivery to human cells.紫外线-DNA修复酶T4内切核酸酶V封装于脂质体并递送至人类细胞。
J Invest Dermatol. 1989 Aug;93(2):190-4. doi: 10.1111/1523-1747.ep12277569.
2
Topical treatment with liposomes containing T4 endonuclease V protects human skin in vivo from ultraviolet-induced upregulation of interleukin-10 and tumor necrosis factor-alpha.含T4核酸内切酶V的脂质体局部治疗可在体内保护人类皮肤免受紫外线诱导的白细胞介素-10和肿瘤坏死因子-α上调的影响。
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Pyrimidine dimer removal enhanced by DNA repair liposomes reduces the incidence of UV skin cancer in mice.DNA修复脂质体增强的嘧啶二聚体去除可降低小鼠紫外线皮肤癌的发病率。
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Pyrimidine dimers in DNA initiate systemic immunosuppression in UV-irradiated mice.DNA中的嘧啶二聚体在紫外线照射的小鼠中引发全身免疫抑制。
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Sunscreens and T4N5 liposomes differ in their ability to protect against ultraviolet-induced sunburn cell formation, alterations of dendritic epidermal cells, and local suppression of contact hypersensitivity.防晒霜和T4N5脂质体在预防紫外线引起的晒伤细胞形成、树突状表皮细胞改变以及局部抑制接触性超敏反应的能力方面存在差异。
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Enhancement of ultraviolet-DNA repair in denV gene transfectants and T4 endonuclease V-liposome recipients.denV基因转染细胞和T4核酸内切酶V-脂质体受体中紫外线-DNA修复的增强
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Effects of sunscreens and a DNA excision repair enzyme on ultraviolet radiation-induced inflammation, immune suppression, and cyclobutane pyrimidine dimer formation in mice.防晒霜和一种DNA切除修复酶对紫外线辐射诱导的小鼠炎症、免疫抑制及环丁烷嘧啶二聚体形成的影响。
J Invest Dermatol. 1993 Oct;101(4):523-7. doi: 10.1111/1523-1747.ep12365902.

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The Skin Microbiome: Is It Affected by UV-induced Immune Suppression?皮肤微生物群：它会受到紫外线诱导的免疫抑制的影响吗？
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