Style Robert W, Boltyanskiy Rostislav, German Guy K, Hyland Callen, MacMinn Christopher W, Mertz Aaron F, Wilen Larry A, Xu Ye, Dufresne Eric R
Yale University, New Haven, CT 06511, USA.
Soft Matter. 2014 Jun 21;10(23):4047-55. doi: 10.1039/c4sm00264d.
Adherent cells, crawling slugs, peeling paint, sessile liquid drops, bearings and many other living and non-living systems apply forces to solid substrates. Traction force microscopy (TFM) provides spatially-resolved measurements of interfacial forces through the quantification and analysis of the deformation of an elastic substrate. Although originally developed for adherent cells, TFM has no inherent size or force scale, and can be applied to a much broader range of mechanical systems across physics and biology. In this paper, we showcase the wide range of applicability of TFM, describe the theory, and provide experimental details and code so that experimentalists can rapidly adopt this powerful technique.
贴壁细胞、爬行的蛞蝓、剥落的油漆、静止的液滴、轴承以及许多其他生物和非生物系统都会对固体基质施加力。牵引力显微镜（TFM）通过对弹性基质变形的量化和分析，提供界面力的空间分辨测量。尽管TFM最初是为贴壁细胞开发的，但它没有固有的尺寸或力标度，并且可以应用于物理和生物学领域更广泛的机械系统。在本文中，我们展示了TFM的广泛适用性，描述了其理论，并提供了实验细节和代码，以便实验人员能够迅速采用这项强大的技术。
