Slais Karel, Stastná Miroslava
Institute of Analytical Chemistry of the Academy of Sciences of the Czech Republic, v. v. i, Brno, Czech Republic.
Electrophoresis. 2014 Sep;35(17):2438-45. doi: 10.1002/elps.201400057. Epub 2014 Jun 12.
In this paper, we suggest new electrolyte system for fast preparative electrofocusing in wide pH range. It is based on bidirectional ITP with multiple counterions and spacers created by commercially available defined simple buffers. The migration course of proposed focusing model can be simulated in advance by using separation conditions and electrolyte components that are consequently applied during the experiments. The suggested electrolyte system allows high current densities at the initial stages of focusing without danger of local overheating, which strongly reduces the time needed for analysis completion. The performance of the electrolyte system is demonstrated by the focusing of synthetic colored low molecular weight indicators and proteins in the arrangements with both linear narrow strip and nonwoven fabric sheet with continuous flow.
在本文中，我们提出了一种用于在宽pH范围内进行快速制备性电聚焦的新型电解质系统。它基于双向等速电泳，使用市售的特定简单缓冲液产生多种抗衡离子和间隔离子。通过使用实验过程中应用的分离条件和电解质成分，可以预先模拟所提出的聚焦模型的迁移过程。所建议的电解质系统在聚焦初始阶段允许高电流密度，而不会有局部过热的危险，这大大减少了完成分析所需的时间。通过在具有线性窄条和连续流动的无纺布片的装置中对合成有色低分子量指示剂和蛋白质进行聚焦，证明了该电解质系统的性能。
