Kim Myeong-Hoon, Kim Bongjune, Lim Eun-Kyung, Choi Yuna, Choi Jihye, Kim Eunjung, Jang Eunji, Park Hyo Seon, Suh Jin-Suck, Huh Yong-Min, Haam Seungjoo
Department of Chemical and Biomolecular Engineering, College of Engineering, Yonsei University, Seoul, 120-749, Republic of Korea.
Macromol Biosci. 2014 Jul;14(7):943-52. doi: 10.1002/mabi.201400029. Epub 2014 Apr 17.
Oleyl dextran-coated magnetic nanoclusters (ODMCs) are fabricated for the accurate detection of macrophage-rich atherosclerotic plaques using magnetic resonance (MR) imaging. Dextran is introduced to the cluster surface of magnetic nanocrystals (MNCs) through self-assembly using amphiphilic oleic acid-conjugated dextran (ODex) to provide not only hydrophilicity but also a high affinity to macrophages. Enhanced magnetism of the ODMCs is engineered by optimizing the degree of substitution (DS) of the oleyl group in ODex and the concentration of ODex used for the synthesis of ODMC. Consequently, ODMCs exhibit significantly increased T2 relaxivity and excellent macrophage-targeting ability without cytotoxicity, in vitro. Moreover, in vivo T2-weighted MR imaging after intravenous injection of ODMCs into a rat artery balloon injury model demonstrates considerable MR contrast strength efficacy in the plaques of the injured carotid artery. These novel ODMCs may offer a highly efficient MR imaging nanoprobes for the selective diagnosis of atherosclerosis.
制备了油酰葡聚糖包被的磁性纳米簇（ODMCs），用于通过磁共振（MR）成像精确检测富含巨噬细胞的动脉粥样硬化斑块。通过使用两亲性油酸共轭葡聚糖（ODex）进行自组装，将葡聚糖引入磁性纳米晶体（MNCs）的簇表面，不仅提供亲水性，还对巨噬细胞具有高亲和力。通过优化ODex中油酰基的取代度（DS）和用于合成ODMC的ODex浓度，设计出增强磁性的ODMCs。因此，ODMCs在体外表现出显著增加的T2弛豫率和优异的巨噬细胞靶向能力，且无细胞毒性。此外，将ODMCs静脉注射到大鼠动脉球囊损伤模型后进行的体内T2加权MR成像显示，在受伤颈动脉的斑块中具有相当大的MR对比增强效果。这些新型ODMCs可能为动脉粥样硬化的选择性诊断提供高效的MR成像纳米探针。
