Tsai Meng-Chen, Chen Mei-Er, Tsai Cheng-Lung
Bachelor Program of Biotechnology, National Chung Hsing University, Taichung, Taiwan.
Arch Insect Biochem Physiol. 2014 Jul;86(3):180-91. doi: 10.1002/arch.21167. Epub 2014 Apr 16.
A Bactrocera dorsalis hexamerin (BdAr) cDNA was cloned (GenBank accession no. KF815528), and its transcriptional expression profiles were determined. The complete 2,530-bp cDNA encodes a 780-amino acid protein with a predicted molecular mass of 94.01 kDa. The proportions of phenylalanine (7.8%), tyrosine (11.2%), and methionine (2.6%) in BdAr as well as all other amino acids are reported. BdAr transcripts were detected in the brain, flight muscle, foregut, Malpighian tubules, and fat body. In the larval stage, BdAr transcripts were expressed in the early third instar and increased in the late third instar. In pupae, the highest expression of BdAr mRNA was present on day 1, then declined and persisted through day 2 to day 8. In adult females, the relative expression of BdAr was significantly higher on day 0 and day 1 compared to day 6 to day 10 while it was highest in newly eclosed adult males. The comparison of the BdAr expression between 8-10-day-old males and females showed a higher level in females. Our phylogenetic analysis results suggest to us that BdAr is similar to Drosophila larval serum protein 1γ.
克隆了桔小实蝇六聚体蛋白（BdAr）的cDNA（基因库登录号：KF815528），并测定了其转录表达谱。完整的2530 bp cDNA编码一个780个氨基酸的蛋白质，预测分子量为94.01 kDa。报告了BdAr以及所有其他氨基酸中苯丙氨酸（7.8%）、酪氨酸（11.2%）和蛋氨酸（2.6%）的比例。在脑、飞行肌、前肠、马氏管和脂肪体中检测到BdAr转录本。在幼虫阶段，BdAr转录本在三龄早期表达，并在三龄晚期增加。在蛹期，BdAr mRNA在第1天表达最高，然后下降并持续到第2天至第8天。在成年雌性中，与第6天至第10天相比，BdAr在第0天和第1天的相对表达显著更高，而在新羽化的成年雄性中最高。8至10日龄雄性和雌性之间BdAr表达的比较显示雌性水平更高。我们的系统发育分析结果表明，BdAr与果蝇幼虫血清蛋白1γ相似。
