Kumar R Sunil, Narasingappa Ramesh Balenahalli, Joshi Chandrashekhar G, Girish Talakatta K, Danagoudar Ananda
Department of Biotechnology, College of Agriculture, University of Agriculture Sciences, Bangalore, Hassan, India.
Department of Studies and Research in Biochemistry, P.G Centre, Mangalore University, Chikka Aluvara, Kodagu, India.
Pharmacogn Mag. 2017 Jul;13(Suppl 2):S250-S257. doi: 10.4103/pm.pm_557_16. Epub 2017 Jul 11.
is a medicinal herb used to cure various ailments in subtropical and tropical regions of Southeast Asia.
The objective of this evaluation of against free radical induced DNA and erythrocyte damage.
The profiles of polyphenol and flavonoid were quantified through reversed-phase high-performance liquid chromatography. Free radical induced DNA and membrane damage were performed using HO as oxidative agent.
The total polyphenol content of leaf ethyl acetate extract (CcEA) was 94.5 ± 3.8 mg/gGAE, CcME ( leaf methanol extract) was 52.7 ± 2.8 mg/gGAE, and CcWE ( leaf Water extract) was 31.84 ± 1.8 mg/gGAE. Total flavonoid content of CcEA was 60.46 ± 2.3 mg/gQE, CcME was 46.26 ± 1.8 mg/gQE, and CcWE was 20.47 ± 1.1 mg/gQE. The extracts also exhibited good antioxidant activity as confirmed by 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2'-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid), hydroxyl scavenging, reducing power, and total antioxidant assays. Among the three extracts, CcEA and CcME showed better protection against red blood cell (RBC) hemolysis and DNA damage as confirmed by electrophoretic study. Further, Scanning electron micrograph data showed that CcEA revealed the free radical induced structural alterations in RBC.
These findings suggest that contains bioactive molecules and can inhibit oxidative stress and can be source of further study to use this in herbal medicine.
ROS are generated under normal biological systems. These ROS generated can be scavenged by endogenous and exogenous cellular mechanisms. Environmental stress, radiation, smoke etc. elevates ROS dramatically. This leads to significant damage to cellular biomolecules like DNA and cell structures. Plants as a large reservoir of drugs for protecting DNA and cell structures from oxidative stress. Polyphenols present in the extracts acts through several mechanisms to quench free radicals. Extracts exhibited potent antioxidant properties and also protected DNA and cell membrane from oxidative damage. Hence this can be used in herbal medicine for treating oxidative stress mediated diseases. ABTS: 2,2'-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid); CcEA: leaf ethyl acetate extract; CcME: leaf methanol extract; CcWE: leaf Water extract; DPPH: 2,2-diphenyl-1-picrylhydrazyl; GAE: Gallic acid Equivalent; HO: Hydrogen Peroxide; QE: Quercetin Equivalent; RNS: Reactive Nitrogen Spevcies; ROS: Reactive Oxygen Species; SEM: Scanning Electron Microscope.
[植物名称]是一种草药，用于治疗东南亚亚热带和热带地区的各种疾病。
本评价旨在研究[植物名称]对自由基诱导的DNA和红细胞损伤的作用。
通过反相高效液相色谱法定量多酚和黄酮类化合物的含量。以过氧化氢作为氧化剂诱导自由基对DNA和细胞膜的损伤。
[植物名称]叶乙酸乙酯提取物（CcEA）的总多酚含量为94.5±3.8mg/g GAE，[植物名称]叶甲醇提取物（CcME）为52.7±2.8mg/g GAE，[植物名称]叶水提取物（CcWE）为31.84±1.8mg/g GAE。CcEA的总黄酮含量为60.46±2.3mg/g QE，CcME为46.26±1.8mg/g QE，CcWE为20.47±1.1mg/g QE。通过2,2-二苯基-1-苦基肼（DPPH）、2,2'-联氮-双（3-乙基苯并噻唑啉-6-磺酸）、羟基清除、还原能力和总抗氧化能力测定，证实提取物具有良好的抗氧化活性。在三种提取物中，电泳研究证实CcEA和CcME对红细胞（RBC）溶血和DNA损伤具有更好的保护作用。此外，扫描电子显微镜数据显示CcEA揭示了自由基诱导的RBC结构改变。
这些发现表明[植物名称]含有生物活性分子，可抑制氧化应激，可作为进一步研究将其用于草药的来源。
活性氧（ROS）在正常生物系统中产生。这些产生的ROS可被内源性和外源性细胞机制清除。环境压力、辐射、烟雾等会显著升高ROS。这会导致对细胞生物分子如DNA和细胞结构的重大损伤。植物作为保护DNA和细胞结构免受氧化应激的药物的大量来源。[植物名称]提取物中存在的多酚通过多种机制清除自由基。提取物表现出强大的抗氧化特性，还能保护DNA和细胞膜免受氧化损伤。因此，它可用于草药治疗氧化应激介导的疾病。ABTS：2,2'-联氮-双（3-乙基苯并噻唑啉-6-磺酸）；CcEA：[植物名称]叶乙酸乙酯提取物；CcME：[植物名称]叶甲醇提取物；CcWE：[植物名称]叶水提取物；DPPH：2,2-二苯基-1-苦基肼；GAE：没食子酸当量；HO：过氧化氢；QE：槲皮素当量；RNS：活性氮物种；ROS：活性氧；SEM：扫描电子显微镜
