Yang Hang, Zhang Yun, Huang Yanling, Yu Junping, Wei Hongping
a Center for Emerging Infectious Diseases and State Key Laboratory of Virology , Wuhan Institute of Virology, Chinese Academy of Sciences , Wuhan , PR China.
Biofouling. 2014;30(6):667-74. doi: 10.1080/08927014.2014.905927. Epub 2014 Apr 17.
Methicillin-resistant Staphylococcus aureus (MRSA) is responsible for a large number of chronic infections due to its ability to form robust biofilms. Herein, the authors evaluated the anti-biofilm activity of a Staphylococcus specific chimeric lysin ClyH on MRSA biofilms. ClyH is known to be active against planktonic MRSA cells in vitro and in vivo. The minimum concentrations for biofilm eradication (MCBE) of ClyH were 6.2-50 mg l(-1), much lower than those of antibiotics. Scanning electron microscope (SEM) analysis revealed that ClyH eliminated MRSA biofilms through cell lytic activity in a time-dependent manner. Viable plate counts and kinetic analysis demonstrated that biofilms of different ages displayed varying susceptibility to ClyH. Together with previously demonstrated in vivo efficacy of ClyH against MRSA, the degradation efficacy against biofilms of different ages indicates that ClyH could be used to remove MRSA biofilms in vivo.
耐甲氧西林金黄色葡萄球菌（MRSA）由于其形成强大生物膜的能力，导致了大量慢性感染。在此，作者评估了一种葡萄球菌特异性嵌合溶素ClyH对MRSA生物膜的抗生物膜活性。已知ClyH在体外和体内对浮游MRSA细胞具有活性。ClyH消除生物膜的最低浓度（MCBE）为6.2 - 50 mg l(-1)，远低于抗生素的浓度。扫描电子显微镜（SEM）分析表明，ClyH通过细胞裂解活性以时间依赖性方式消除MRSA生物膜。活菌平板计数和动力学分析表明，不同年龄的生物膜对ClyH表现出不同的敏感性。连同先前证明的ClyH对MRSA的体内疗效，对不同年龄生物膜的降解效果表明ClyH可用于在体内去除MRSA生物膜。
