University of Veterinary Medicine and Pharmacy, SK 041 81 Kosice, Slovakia.
Institute of Virology, Department of Infectious Diseases, University of Veterinary Medicine, D-30559 Hannover, Germany.
Vet Microbiol. 2014 Jun 25;171(1-2):87-92. doi: 10.1016/j.vetmic.2014.03.028. Epub 2014 Mar 30.
For the pestivirus border disease virus (BDV) at least seven major genotypes have been described (BDV-1-BDV-7). So far, complete genomic sequences have been reported for four BDV genotypes (BDV-1-BDV-4). In this study we report the entire genomic sequence of the noncytopathogenic (ncp) BDV-5 reference strain Aveyron. The viral genome encompasses 12,284 nucleotides (nt) and contains one large open reading frame (11,700 nt) flanked by a 370 nt long 5'-untranslated region (UTR) and a 214 nt long 3'-UTR. The genome organization as well as the lengths of the viral polyprotein (3899 amino acids) and the 5'-UTR are very similar to the ones of other BDV strains, while the 3'-UTR of BDV Aveyron is considerably shorter when compared to other BDV strains. Comparative analysis of complete coding sequences revealed that BDV Aveyron shares nucleotide sequence identities of 76.9% to 79.0% with the other BDV strains, and less than 72% identity with other pestiviruses. In contrast to other BDV strains, a unique insertion of four amino acids (KAPD) of unknown origin is present in the C-terminal part of the viral autoprotease NS2 encoded by BDV Aveyron. Immunoblot analysis revealed that infection of cells with the ncp BDV strain Aveyron comprising this unique insertion in NS2 resulted in the expression of high amounts of NS3 and thereby showed that BDV Aveyron significantly differs from other ncp BDV strains in terms of NS2-3 processing and production of NS3.
对于边界病病毒（BDV）至少已经描述了七个主要基因型（BDV-1-BDV-7）。到目前为止，已经报道了四个 BDV 基因型（BDV-1-BDV-4）的完整基因组序列。在这项研究中，我们报告了非细胞病变性（ncp）BDV-5 参考株 Aveyron 的整个基因组序列。病毒基因组包含 12284 个核苷酸（nt），并包含一个由 370nt 长的 5'-非翻译区（UTR）和 214nt 长的 3'-UTR 侧翼的 11700nt 长的大开放阅读框（ORF）。基因组组织以及病毒多蛋白（3899 个氨基酸）和 5'-UTR 的长度与其他 BDV 株非常相似，而与其他 BDV 株相比，BDV Aveyron 的 3'-UTR 要短得多。完整编码序列的比较分析表明，BDV Aveyron 与其他 BDV 株的核苷酸序列同一性为 76.9%-79.0%，与其他瘟病毒的同一性小于 72%。与其他 BDV 株不同的是，在由 BDV Aveyron 编码的病毒自蛋白酶 NS2 的 C 末端存在一个独特的插入四个氨基酸（KAPD）的未知来源。免疫印迹分析显示，用包含 NS2 中这种独特插入的 ncp BDV 株 Aveyron 感染细胞会导致 NS3 的大量表达，从而表明 BDV Aveyron 在 NS2-3 加工和 NS3 产生方面与其他 ncp BDV 株明显不同。
