MacGillivray A J, Allday M J, Saunders S E, Sinclair J H
School of Biological Sciences, University of Sussex, Brighton, UK.
Br J Cancer Suppl. 1988 Dec;9:93-7.
EBNA-1 is a nuclear antigen of lymphocytes infected by Epstein-Barr virus and whose size polymorphism correlates only with the strain of infecting virus and the length of the glycine-alanine copolymer encoded by the third internal repeat of the viral genome. The major antigenic determinant(s) also appear to reside in this region. We have been able to obtain efficient expression of this nuclear antigen in cultured Drosophila cells transfected with a cosmid carrying the EBNA-1 coding region, indicating that insect mechanisms recognise control sequences and transcripts of the herpes virus. The association of a vimertin-like protein of mol. wt. 46,000 with Drosophila cell nuclei has been found to vary with culture conditions and heat shock. We now find that the level and nuclear association of this protein also increase after transfection with either EBNA-1 or yolk protein DNA.
EBNA-1是受爱泼斯坦-巴尔病毒感染的淋巴细胞的一种核抗原，其大小多态性仅与感染病毒的毒株以及病毒基因组第三个内部重复序列编码的甘氨酸-丙氨酸共聚物的长度相关。主要抗原决定簇似乎也位于该区域。我们已经能够在用携带EBNA-1编码区的黏粒转染的培养果蝇细胞中高效表达这种核抗原，这表明昆虫机制能够识别疱疹病毒的调控序列和转录本。已发现一种分子量为46,000的类波形蛋白与果蝇细胞核的结合会随培养条件和热休克而变化。我们现在发现，在用EBNA-1或卵黄蛋白DNA转染后，这种蛋白的水平及其与细胞核的结合也会增加。
