Hammarstrom S, Shively J E, Paxton R J, Beatty B G, Larsson A, Ghosh R, Bormer O, Buchegger F, Mach J P, Burtin P
University of Umeå.
Cancer Res. 1989 Sep 1;49(17):4852-8.
The epitope reactivities of 52 well-characterized monoclonal antibodies (Mabs) against carcinoembryonic antigen from 11 different research groups were studied using competitive solid-phase immunoassays. About 60% of all possible combinations of Mabs as inhibitors and as the primary binding antibody were investigated. The inhibition data were analyzed by a specially developed computer program "EPITOPES" which measures concordance and discordance in inhibition patterns between Mabs. The analysis showed that 43 of the 52 Mabs (83%) could be classified into one of five essentially noninteracting epitope groups (GOLD 1-5) containing between four and 15 Mabs each. The epitopes recognized by the Mabs belonging to groups 1 to 5 were peptide in nature. With one or two possible exceptions non-classifiable Mabs were either directed against carbohydrate epitopes (4 Mabs) or were inactive in the tests used. Within epitope groups GOLD 1, 4, and 5 two partially overlapping subgroups were distinguished. Mabs with a high degree of carcinoembryonic antigen specificity generally belonged to epitope groups GOLD 1 and 3.
使用竞争性固相免疫测定法研究了来自11个不同研究小组的52种特征明确的抗癌胚抗原单克隆抗体（Mab）的表位反应性。研究了约60%的Mab作为抑制剂和作为主要结合抗体的所有可能组合。抑制数据通过专门开发的计算机程序“EPITOPES”进行分析，该程序测量Mab之间抑制模式的一致性和不一致性。分析表明，52种Mab中的43种（83%）可分为五个基本不相互作用的表位组（GOLD 1-5）之一，每个表位组包含4至15种Mab。属于第1至5组的Mab识别的表位本质上是肽。除了一两个可能的例外，无法分类的Mab要么针对碳水化合物表位（4种Mab），要么在所用测试中无活性。在表位组GOLD 1、4和5中，区分出两个部分重叠的亚组。具有高度癌胚抗原特异性的Mab通常属于表位组GOLD 1和3。
