利用微小RNA将体细胞重编程为多能干细胞。

Reprogramming somatic cells into pluripotent stem cells using miRNAs.

作者信息

Anokye-Danso Frederick

机构信息

Department of Medicine, Perelman School of Medicine, University of Pennsylvania, 3400 Civic Center Boulevard, Bldg 421, Philadelphia, PA, 19104, USA,

出版信息

Methods Mol Biol. 2014;1150:273-81. doi: 10.1007/978-1-4939-0512-6_19.

DOI:10.1007/978-1-4939-0512-6_19

PMID:24744006

Abstract

Reversal of terminally differentiated somatic cells to ground-state pluripotency has rejuvenated our hopes of generating patient-specific stem cells for therapeutic use in regenerative medicine and drug screening. Originally generated using defined exogenous protein-coding DNA, several methods have been described in reprogramming somatic cells into iPSC. Majority of published methods seek to improve or refine the techniques of reprogramming. This chapter describes reprogramming to pluripotency using miRNAs.

摘要

将终末分化的体细胞逆转为基础状态多能性，重燃了我们为再生医学和药物筛选生成患者特异性干细胞用于治疗的希望。最初是使用特定的外源性蛋白质编码DNA生成的，目前已有多种将体细胞重编程为诱导多能干细胞（iPSC）的方法被描述。大多数已发表的方法旨在改进或完善重编程技术。本章介绍了使用微小RNA（miRNA）将细胞重编程为多能性的方法。