Dhurandhar N R, Zorrilla O
Department of Pathology, Tulane University Medical Center, New Orleans, LA 70112.
Diagn Cytopathol. 1988;4(4):347-51. doi: 10.1002/dc.2840040415.
We describe a technique for performing special stains on one half of a slide previously stained by the Papanicolaou or Diff-Quick method. The technique varies depending on whether incubation is required during the staining procedure. For stains that do not require incubation, the portion of the slide to be preserved is covered with liquid paraffin. For stains that do require incubation, the area to be preserved is coverslipped with mounting medium and dried overnight prior to staining. The technique is easy, rapid, inexpensive, and enhances diagnostic accuracy.
我们描述了一种对先前用巴氏或Diff-Quick方法染色的玻片的一半进行特殊染色的技术。该技术根据染色过程中是否需要孵育而有所不同。对于不需要孵育的染色，要用液体石蜡覆盖玻片上要保留的部分。对于需要孵育的染色，在染色前，要用封片剂覆盖要保留的区域并干燥过夜。该技术简便、快速、成本低，并能提高诊断准确性。
