Nose T, Tsurumi K, Kawada K, Nagai H, Yamada H, Yakuo I, Ojima A, Koda A
Department of Pharmacology, Gifu University School of Medicine, Japan.
Inflammation. 1989 Aug;13(4):401-14. doi: 10.1007/BF00914924.
In order to characterize nephrotoxic serum nephritis accelerated with rabbit gamma-globulin in mice, histopathological studies were carried out 15 days after NTS injection, the time when increases in urinary protein and serum cholesterol and a decrease in serum albumin were apparent. Characteristic changes were widespread thickening of glomerular capillary walls and widening of mesangial areas, owing to deposits of mesangial matrixlike substances. The mesangial interposition into subendothelial areas and the resultant narrowing of the capillary lumen were shown ultrastructurally. In severely affected glomeruli, a hyaline nodular lesion was observed. Visceral epithelial cells demonstrated fusion of the foot processes, microvilli formation, occasional proliferation, and enlargement. Parietal epithelial cells proliferated, forming a cellular crescent. Based on these characteristics, it appears this nephritic model shares a common pathology with human membranoproliferative glomerulonephritis type 1 and crescentic glomerulonephritis and can be considered an appropriate model for producing severe nephritis for short periods.
为了描述用兔γ球蛋白加速诱导的小鼠肾毒性血清肾炎的特征，在注射肾毒性血清（NTS）15天后进行了组织病理学研究，此时尿蛋白、血清胆固醇升高以及血清白蛋白降低的情况较为明显。特征性变化包括肾小球毛细血管壁广泛增厚和系膜区增宽，这是由于系膜样基质物质沉积所致。超微结构显示系膜插入内皮下区域，导致毛细血管腔狭窄。在严重受累的肾小球中，观察到透明结节性病变。脏层上皮细胞表现为足突融合、微绒毛形成、偶尔增殖和肿大。壁层上皮细胞增殖，形成细胞性新月体。基于这些特征，这种肾炎模型似乎与人类1型膜增生性肾小球肾炎和新月体性肾小球肾炎具有共同的病理学特征，可被认为是短期内产生严重肾炎的合适模型。
