不同生物工厂中重组蛋白生产的比较评估：绿色视角。
Comparative evaluation of recombinant protein production in different biofactories: the green perspective.
机构信息
Department of Biotechnology, University of Verona, 37134 Verona, Italy.
出版信息
Biomed Res Int. 2014;2014:136419. doi: 10.1155/2014/136419. Epub 2014 Mar 12.
Abstract
In recent years, the production of recombinant pharmaceutical proteins in heterologous systems has increased significantly. Most applications involve complex proteins and glycoproteins that are difficult to produce, thus promoting the development and improvement of a wide range of production platforms. No individual system is optimal for the production of all recombinant proteins, so the diversity of platforms based on plants offers a significant advantage. Here, we discuss the production of four recombinant pharmaceutical proteins using different platforms, highlighting from these examples the unique advantages of plant-based systems over traditional fermenter-based expression platforms.
摘要
近年来，异源系统中重组药物蛋白的生产显著增加。大多数应用涉及难以生产的复杂蛋白质和糖蛋白，从而促进了广泛的生产平台的发展和改进。没有一个单一的系统对所有重组蛋白的生产都是最优的，因此基于植物的平台多样性具有显著的优势。在这里，我们讨论了使用不同平台生产四种重组药物蛋白的情况，从这些例子中突出了植物基系统相对于传统发酵器表达平台的独特优势。
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本文引用的文献
1
Comparative analysis of different biofactories for the production of a major diabetes autoantigen.
Transgenic Res. 2014 Apr;23(2):281-91. doi: 10.1007/s11248-013-9749-9. Epub 2013 Oct 20.
2
Targeting interleukin-6 in inflammatory autoimmune diseases and cancers.
Pharmacol Ther. 2014 Feb;141(2):125-39. doi: 10.1016/j.pharmthera.2013.09.004. Epub 2013 Sep 27.
3
Interleukin-6 polymorphisms and hematologic malignancy: a re-appraisal of evidence from genetic association studies.
Biomarkers. 2013 Nov;18(7):625-31. doi: 10.3109/1354750X.2013.840799. Epub 2013 Sep 23.
4
The role of interleukin-6 in the evolution of ovarian cancer: clinical and prognostic implications--a review.
J Mol Med (Berl). 2013 Dec;91(12):1355-68. doi: 10.1007/s00109-013-1080-7. Epub 2013 Sep 21.
5
Expression and assembly of Norwalk virus-like particles in plants using a viral RNA silencing suppressor gene.
Appl Microbiol Biotechnol. 2013 Oct;97(20):9021-7. doi: 10.1007/s00253-013-5077-5. Epub 2013 Aug 8.
6
Oral delivery of therapeutic proteins and peptides: a review on recent developments.
Drug Deliv. 2013 Aug;20(6):237-46. doi: 10.3109/10717544.2013.819611.
7
Plant species and organ influence the structure and subcellular localization of recombinant glycoproteins.
Plant Mol Biol. 2013 Sep;83(1-2):105-17. doi: 10.1007/s11103-013-0049-9. Epub 2013 Apr 4.
8
Rapid high-level production of functional HIV broadly neutralizing monoclonal antibodies in transient plant expression systems.
PLoS One. 2013;8(3):e58724. doi: 10.1371/journal.pone.0058724. Epub 2013 Mar 22.
9
Optimizing the yield of recombinant pharmaceutical proteins in plants.
Curr Pharm Des. 2013;19(31):5486-94. doi: 10.2174/1381612811319310004.
10
Commercial aspects of pharmaceutical protein production in plants.
Curr Pharm Des. 2013;19(31):5471-7. doi: 10.2174/1381612811319310002.
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