Department of Neurosurgery, Mackay Memorial Hospital, Taipei 104, Taiwan ; Graduate Institute of Injury Prevention and Control, Taipei Medical University, Taipei 110, Taiwan.
Department of Cardiology, College of Medicine, Wan Fang Hospital, Taipei Medical University, Taipei 116, Taiwan.
Biomed Res Int. 2014;2014:484161. doi: 10.1155/2014/484161. Epub 2014 Mar 17.
The present study investigated the merit of ginseng in the improvement of heart failure in diabetic rats and the role of peroxisome proliferator-activated receptors δ (PPAR δ ). We used streptozotocin-induced diabetic rat (STZ-rat) to screen the effects of ginseng on cardiac performance and PPAR δ expression. Changes of body weight, water intake, and food intake were compared in three groups of age-matched rats; the normal control (Wistar rats) received vehicle, STZ-rats received vehicle and ginseng-treated STZ-rats. We also determined cardiac performances in addition to blood glucose level in these animals. The protein levels of PPAR δ in hearts were identified using Western blotting analysis. In STZ-rats, cardiac performances were decreased but the food intake, water intake, and blood glucose were higher than the vehicle-treated control. After a 7-day treatment of ginseng in STZ-rats, cardiac output was markedly enhanced without changes in diabetic parameters. This treatment with ginseng also increased the PPAR δ expression in hearts of STZ-rats. The related signal of cardiac contractility, troponin I phosphorylation, was also raised. Ginseng-induced increasing of cardiac output was reversed by the cotreatment with PPAR δ antagonist GSK0660. Thus, we suggest that ginseng could improve heart failure through the increased PPAR δ expression in STZ-rats.
本研究探讨了人参在改善糖尿病大鼠心力衰竭中的作用及其与过氧化物酶体增殖物激活受体 δ（PPAR δ）的关系。我们使用链脲佐菌素诱导的糖尿病大鼠（STZ-大鼠）筛选人参对心脏功能和 PPAR δ 表达的影响。比较三组年龄匹配的大鼠的体重、饮水量和采食量变化；正常对照组（Wistar 大鼠）给予载体，STZ 大鼠给予载体和人参处理的 STZ 大鼠。我们还测定了这些动物的心脏功能以及血糖水平。使用 Western blot 分析鉴定心脏中 PPAR δ 的蛋白水平。在 STZ 大鼠中，心脏功能降低，但食物摄入、水摄入和血糖高于载体处理的对照组。在 STZ 大鼠中用人参治疗 7 天后，心输出量明显增加，而糖尿病参数无变化。这种人参治疗还增加了 STZ 大鼠心脏中的 PPAR δ 表达。心肌收缩力相关信号肌钙蛋白 I 磷酸化也升高。用 PPAR δ 拮抗剂 GSK0660 共同处理可逆转人参诱导的心输出量增加。因此，我们认为人参可以通过增加 STZ 大鼠的 PPAR δ 表达来改善心力衰竭。
