Muller S, Bonnier D, Thiry M, Van Regenmortel M H
Institut de Biologie Moléculaire et Cellulaire, CNRS, Strasbourg, France.
Int Arch Allergy Appl Immunol. 1989;89(2-3):288-96. doi: 10.1159/000234962.
The specificity of autoantibodies present in the serum of 151 patients with systemic lupus erythematosus (SLE) was investigated by ELISA using as antigen individual histones as well as 17 different core histone synthetic peptides. Many of the sera reacted with four terminal peptides (residues 1-21 and 130-135 of H3, 1-29 of H4 and 1-25 of H2B) while fewer reacted with internal peptides (residues 65-85 of H2A and 40-55 of H3). Of the 151 SLE sera, 88% reacted with one or more of the six core histone peptides whereas only 57% reacted with one or more of the complete core histone molecules. Antibodies to mononucleosomes from chicken erythrocytes were also prepared in rabbits. The rabbit antisera were tested by ELISA using as antigen chromatin subunits, native and denatured DNA, individual histones and 23 natural and synthetic peptides of histones. The antinucleosome antibodies were found to recognize the same peptide fragments as those recognized by the SLE sera.
采用酶联免疫吸附测定（ELISA），以单个组蛋白以及17种不同的核心组蛋白合成肽作为抗原，对151例系统性红斑狼疮（SLE）患者血清中自身抗体的特异性进行了研究。许多血清与4种末端肽发生反应（H3的第1 - 21位和第130 - 135位、H4的第1 - 29位以及H2B的第1 - 25位），而与内部肽发生反应的血清较少（H2A的第65 - 85位和H3的第40 - 55位）。在151份SLE血清中，88%与6种核心组蛋白肽中的一种或多种发生反应，而只有57%与一种或多种完整的核心组蛋白分子发生反应。还在兔体内制备了针对鸡红细胞单核小体的抗体。用ELISA对兔抗血清进行检测，所用抗原为染色质亚基、天然和变性DNA、单个组蛋白以及23种组蛋白的天然和合成肽。发现抗核小体抗体识别的肽片段与SLE血清识别的相同。
