Dulińska-Molak Ida, Mao Hongli, Kawazoe Naoki, Chen Guoping
J Nanosci Nanotechnol. 2014 Mar;14(3):2459-65. doi: 10.1166/jnn.2014.8529.
It is important to elucidate the effects of carbon nanotubes on cell functions for their biomedical applications. In this study, the effect of single-walled carbon nanotubes (SWCNTs) on the mechanical property of chondrocytes was investigated by atomic force microscopy. Chondrocytes were cultured in medium containing SWCNTs and showed an increase uptake of SWCNTs with culture time. The mechanical property of chondrocytes cultured with or without SWCNTs was measured at an indentation depth of 200 nm and 500 nm. The chondrocytes cultured with SWCNTs showed higher Young's modulus than that of cells cultured without SWCNTs at both indentation depths. The increase became significant after culture for more than 3 hours. Indentation at 500 nm depth magnified the change of Young's modulus compared to that monitored at 200 nm indentation depth. The results indicated uptake of SWCNTs increased the Young's modulus of chondrocytes.
阐明碳纳米管在生物医学应用中对细胞功能的影响很重要。在本研究中，通过原子力显微镜研究了单壁碳纳米管（SWCNT）对软骨细胞力学性能的影响。软骨细胞在含有SWCNT的培养基中培养，并且随着培养时间的增加对SWCNT的摄取量增加。在200nm和500nm的压痕深度下测量了添加或未添加SWCNT培养的软骨细胞的力学性能。在两个压痕深度下，添加SWCNT培养的软骨细胞均显示出比未添加SWCNT培养的细胞更高的杨氏模量。培养超过3小时后，这种增加变得显著。与在200nm压痕深度下监测的结果相比，500nm深度的压痕放大了杨氏模量的变化。结果表明，SWCNT的摄取增加了软骨细胞的杨氏模量。
