导电导管可抑制细菌定植。
Electrically conductive catheter inhibits bacterial colonization.
作者信息
Amalou Hayet, Negussie Ayele H, Ranjan Ashish, Chow Lucy, Xu Sheng, Kroeger Craig, Neeman Ziv, O'Grady Naomi P, Wood Bradford J
机构信息
Center for Interventional Oncology, Clinical Center and National Cancer Institute, National Institutes of Health, Building 10, MSC 1182, Bethesda, MD 20892-1182.
VitalDyne, Inc, Cokato, Minnesota.
出版信息
J Vasc Interv Radiol. 2014 May;25(5):797-802. doi: 10.1016/j.jvir.2014.01.032.
PURPOSE
To design, prototype, and assess a custom vascular access catheter for its ability to inhibit bacterial colonization in vitro and to optimize electric parameters for efficacy and safe translation.
MATERIALS AND METHODS
A vascular access catheter with conductive elements was designed and custom fabricated with two electrodes at the tip, separated by a nonconductive segment. The catheter was colonized with Staphylococcus aureus and incubated at predetermined current levels (4-8 µA) and durations (4-24 h). Catheters were compared using bacterial counts and scanning electron microscopy (SEM).
RESULTS
Bacteria colony-forming units were reduced significantly (P < .05) by > 90% (91.7%-100%) in all uninterrupted treatment arms that included electric current (4 µA or 8 µA) of at least 8 hours' duration. Qualitative analysis using SEM revealed that the treated catheter exposed to electric current had markedly less bacteria compared with the untreated catheter.
CONCLUSIONS
This catheter with conductive elements inhibits bacterial colonization in vitro when very small electric current (4-8 µA) is applied across the tip for 8-24 hours. In vivo validation is requisite to future translation to the clinical setting.
目的
设计、制作定制血管通路导管的原型，并评估其在体外抑制细菌定植的能力，优化电参数以确保疗效和安全应用。
材料与方法
设计并定制了一种带有导电元件的血管通路导管，其尖端有两个电极，由一个非导电段隔开。将金黄色葡萄球菌接种到导管上，并在预定的电流水平（4 - 8 μA）和持续时间（4 - 24小时）下进行培养。使用细菌计数和扫描电子显微镜（SEM）对导管进行比较。
结果
在所有持续至少8小时电流（4 μA或8 μA）的不间断治疗组中，细菌集落形成单位显著减少（P < 0.05），减少幅度超过90%（91.7% - 100%）。使用SEM进行的定性分析表明，与未处理的导管相比，通电处理的导管上的细菌明显更少。
结论
当在导管尖端施加非常小的电流（4 - 8 μA）持续8 - 24小时时，这种带有导电元件的导管在体外可抑制细菌定植。未来向临床应用转化需要进行体内验证。
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