Reiriz J, Mena M A, Bazán E, Muradás V, Lerma J, Delgado J M, De Yébenes J G
Departamento de Investigación, Centro Especial Ramón y Cajal, Madrid, Spain.
J Neurochem. 1989 Sep;53(3):789-92. doi: 10.1111/j.1471-4159.1989.tb11774.x.
We have measured the levels of monoamines and their metabolites in rat striata implanted with a dialysis tube, in contralateral nonimplanted striata, and in dialysates obtained from the dialysis tube. The perfusion was done with Ringer solution. The animals were perfused either for a continuous period of 7 h at 1 day after implantation or for periods of 2 h on days 1, 4, and 7 after implantation. In animals perfused for 7 h, levels of monoamine metabolites in dialysates remained stable for the first 4 h of perfusion, but a reduction was observed during the last 3 h. In animals perfused for 2 h on days 1, 4, and 7 after implantation, we observed a progressive reduction in levels of metabolites in dialysates with respect to the first day of perfusion. The levels of dopamine and its metabolites in the striata in which a dialysis cannula had been implanted showed a progressive reduction during the period postimplantation comparable to that observed in dialysates. The levels of 3-methoxytyramine, 3,4-dihydroxyphenylacetic acid, and homovanillic acid were elevated 24 h after implantation in the implanted striata with respect to the contralateral nonimplanted striata, but 7 days after implantation, the levels of dopamine were decreased in the implanted striata, and the levels of metabolites were unchanged.
我们测量了植入透析管的大鼠纹状体、对侧未植入的纹状体以及从透析管获得的透析液中的单胺及其代谢物水平。灌注使用林格氏液进行。动物在植入后1天连续灌注7小时，或在植入后第1、4和7天分别灌注2小时。在灌注7小时的动物中，透析液中单胺代谢物水平在灌注的前4小时保持稳定，但在最后3小时出现下降。在植入后第1、4和7天灌注2小时的动物中，我们观察到透析液中代谢物水平相对于灌注第一天逐渐降低。植入透析套管的纹状体中多巴胺及其代谢物水平在植入后期间逐渐降低，与透析液中观察到的情况相当。与对侧未植入的纹状体相比，植入的纹状体中3-甲氧基酪胺、3,4-二羟基苯乙酸和高香草酸水平在植入后24小时升高，但在植入7天后，植入的纹状体中多巴胺水平降低，代谢物水平未变。
