Shimoyama M, Kito S, Inokawa M
Third Department of Internal Medicine, Hiroshima University School of Medicine, Japan.
Arzneimittelforschung. 1988 Feb;38(2):243-7.
The effect of a xanthine derivative, 3-methyl-1-(5-oxohexyl)-7-propylxanthine (HWA 285, 10 mg/kg i.p.), on the release of endogenous monoamines and their metabolites from the rat striatum and hippocampus are examined in vivo by intracerebral dialysis technique. Using this method, which is based on the dialysis principle, a small-calibered (0.5 mm) dialysis tube is inserted into the striatum or hippocampus transversally and is perfused with Ringer solution. The amounts of dopamine, 3,4-dihydroxyphenylacetic acid, homovanillic acid, 5-hydroxytryptamine and 5-hydroxyindoleacetic acid in the perfusates are measured by high performance liquid chromatography system with electrochemical detection. HWA 285 (10 mg/kg) decreased monoamine levels in hippocampal perfusates after its i.p. administration. However, there were no consistent changes in monoamine release in striatal perfusates after the drug administration. These findings suggest that HWA 285 exerts an inhibitory influence on hippocampal dopaminergic and serotonergic systems.
采用脑内透析技术在体内研究黄嘌呤衍生物3-甲基-1-(5-氧代己基)-7-丙基黄嘌呤（HWA 285，10毫克/千克腹腔注射）对大鼠纹状体和海马体内源性单胺及其代谢产物释放的影响。使用基于透析原理的该方法，将一根小口径（0.5毫米）透析管横向插入纹状体或海马，并灌注林格溶液。通过具有电化学检测功能的高效液相色谱系统测量灌注液中多巴胺、3,4-二羟基苯乙酸、高香草酸、5-羟色胺和5-羟吲哚乙酸的含量。腹腔注射HWA 285（10毫克/千克）后，海马灌注液中的单胺水平降低。然而，给药后纹状体灌注液中的单胺释放没有一致变化。这些发现表明，HWA 285对海马多巴胺能和5-羟色胺能系统具有抑制作用。
