Yasuda K, Aiso S, Nagatani T, Yamada M
Department of Anatomy, School of Medicine, Keio University, Tokyo, Japan.
J Electron Microsc Tech. 1989 Jun;12(2):155-9. doi: 10.1002/jemt.1060120209.
Using an ultrahigh-resolution scanning electron microscope (SEM), an attempt was made to directly visualize antibody molecules at antigenic sites in rat pancreas or on silicone plates. Although individual antibody molecules could not be discerned, a fluffy meshwork, probably indicating several molecules, was seen. With further improvements in specimen preparation, the high-resolution SEM promises to be an important tool in examining individual antibody-antigen sites without the need of an electron-dense label such as colloidal gold.
使用超高分辨率扫描电子显微镜（SEM），尝试直接观察大鼠胰腺或硅胶板抗原位点处的抗体分子。虽然无法辨别单个抗体分子，但可以看到一个蓬松的网络结构，可能表明存在多个分子。随着样品制备技术的进一步改进，高分辨率SEM有望成为一种无需胶体金等电子致密标记物就能检测单个抗体-抗原位点的重要工具。
